caa a22 4500 469028467 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00232537 doi (NATIONALLICENCE)springer-10.1007/BF00232537 Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L. ssp. Oleifera [Elektronische Daten] [Laurie Burnett, Stephen Yarrow, Bin Huang] Summary: Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspore embryogenesis, buds (2-3mm in length, containing mid-late uninucieate microspores) were collected from older plants (2 months old) and microspores isolated and washed in iron-free B5 medium. NLN medium with its iron content reduced to half was beneficial for initial microspore culture. An elevated temperature(33-35°C) during the first day of culture, followed by maintenance at 25°C resulted in dozens of embryos from each isolation (about 100 buds). Seeds were obtained from plants regenerated from microsporederived embryos after colchicine treatment. Springer-Verlag, 1992 Microspore embryogenesis nationallicence B.rapa nationallicence genotypes nationallicence iron toxicity nationallicence Burnett Laurie Allelix Crop Technologies (A division of Pioneer Hi-Bred Production Ltd), 6850 Goreway Dr., L4V 1P1, Mississauga, Ont., Canada aut Yarrow Stephen Allelix Crop Technologies (A division of Pioneer Hi-Bred Production Ltd), 6850 Goreway Dr., L4V 1P1, Mississauga, Ont., Canada aut Huang Bin Allelix Crop Technologies (A division of Pioneer Hi-Bred Production Ltd), 6850 Goreway Dr., L4V 1P1, Mississauga, Ont., Canada aut Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 215-218 0721-7714 11:4<215 1992 11 299 https://doi.org/10.1007/BF00232537 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232537 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Burnett Laurie Allelix Crop Technologies (A division of Pioneer Hi-Bred Production Ltd), 6850 Goreway Dr., L4V 1P1, Mississauga, Ont., Canada aut NATIONALLICENCE 700 1- Yarrow Stephen Allelix Crop Technologies (A division of Pioneer Hi-Bred Production Ltd), 6850 Goreway Dr., L4V 1P1, Mississauga, Ont., Canada aut NATIONALLICENCE 700 1- Huang Bin Allelix Crop Technologies (A division of Pioneer Hi-Bred Production Ltd), 6850 Goreway Dr., L4V 1P1, Mississauga, Ont., Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 215-218 0721-7714 11:4<215 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer