caa a22 4500 469028491 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00232535 doi (NATIONALLICENCE)springer-10.1007/BF00232535 Organogenesis and a general procedure for plant regeneration from callus culture of a commercial duboisia hybrid ( D. leichhardtii x D. myoporoides ) [Elektronische Daten] [G. Lin, W. Griffin] Summary: The Duboisia hybrid (D. leichhardtii x D. myoporoides) was regenerated from callus derived from shoot tips and young seeds on Murashige and Skoog (MS) medium containing 54μM 1-naphthalenacetic acid (NAA) and 1μM 6-benzylaminopurine (BA). Callus derived from young seeds was superior to shoot tip callus. Buds were induced from callus on MS medium supplemented with 22μM BA. The buds formed shoots when transferred to MS medium with 5 μM BA and 0.5μM NAA. Shoots were induced to form roots when placed on MS medium containing 25 μM indolebutyric acid (IBA). Plantlets so formed were transplanted and subsequently planted out in the open. Approximately 6 months were required for the full regenerative process. Trees so formed have been grown for 3 years, reached a height of 3 metres and flowered and fruited normally. Springer-Verlag, 1992 Duboisia hybrid nationallicence callus culture nationallicence organogenesis nationallicence regeneration nationallicence Lin G. Department of Pharmacy, University of Queensland, 4072, Queensland, Australia aut Griffin W. Department of Pharmacy, University of Queensland, 4072, Queensland, Australia aut Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 207-210 0721-7714 11:4<207 1992 11 299 https://doi.org/10.1007/BF00232535 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00232535 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin G. Department of Pharmacy, University of Queensland, 4072, Queensland, Australia aut NATIONALLICENCE 700 1- Griffin W. Department of Pharmacy, University of Queensland, 4072, Queensland, Australia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/4(1992-05-01), 207-210 0721-7714 11:4<207 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer