caa a22 4500 469028513 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00234370 doi (NATIONALLICENCE)springer-10.1007/BF00234370 Plant regeneration from Carica protoplasts [Elektronische Daten] [M. Chen, C. Chen] Summary: Rapidly proliferating and highly regenerable suspension cultures of somatic embryos of Carica papaya x C. cauliflora were used for protoplast isolation. On average, protoplast yield was 1.5×106/g fresh weight of somatic embryos. Protoplasts were first cultured in liquid KM8P-S medium for 2 weeks and then plated in the same medium solidified with 1% agarose. About 1.4% of the protoplasts developed directly into somatic embryos. Protoplast-derived somatic embryos proliferated rapidly through direct embryogenesis on modified MS medium supplemented with 1 mg/1 ABA, and developed into plantlets upon transfer to MS medium devoid of plant growth regulators. The plantlets were successfully transplanted to soil. Springer-Verlag, 1992 Chen M. Department of Botany, National Taiwan University, Taipei, Taiwan 107, Republic of China aut Chen C. Department of Botany, National Taiwan University, Taipei, Taiwan 107, Republic of China aut Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 404-407 0721-7714 11:8<404 1992 11 299 https://doi.org/10.1007/BF00234370 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00234370 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen M. Department of Botany, National Taiwan University, Taipei, Taiwan 107, Republic of China aut NATIONALLICENCE 700 1- Chen C. Department of Botany, National Taiwan University, Taipei, Taiwan 107, Republic of China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 404-407 0721-7714 11:8<404 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer