caa a22 4500 469028521 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00234368 doi (NATIONALLICENCE)springer-10.1007/BF00234368 The effect of temperature on hairy root cultures of Catharanthus roseus: Growth, indole alkaloid accumulation and membrane lipid composition [Elektronische Daten] [Leena Toivonen, Simo Laakso, Heikki Rosenqvist] Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to an increased proportion of linolenic acid in the main lipid classes. The modifications in lipid composition were obviously necessary for the roots to retain the proper cell membrane fluidity at each temperature. Despite of changes in membrane lipids, no effect on the distribution of indole alkaloids between the roots and the medium could be detected. Instead, the level of alkaloid accumulation showed a clear increase with lowering temperature. Springer-Verlag, 1992 Toivonen Leena Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut Laakso Simo Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut Rosenqvist Heikki Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 395-399 0721-7714 11:8<395 1992 11 299 https://doi.org/10.1007/BF00234368 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00234368 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toivonen Leena Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut NATIONALLICENCE 700 1- Laakso Simo Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut NATIONALLICENCE 700 1- Rosenqvist Heikki Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 395-399 0721-7714 11:8<395 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer