caa a22 4500 46902853X CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00234375 doi (NATIONALLICENCE)springer-10.1007/BF00234375 Establishment of hairy root cultures of Psoralea species [Elektronische Daten] [Christophe Nguyen, Frédéric Bourgaud, Paul Forlot, Armand Guckert] Eight Psoralea species (Leguminosae) were inoculated with Agrobacterium rhizogenes, strains 8196 and 9402. Hairy roots were only induced by strain 9402. Attention was focussed on Psoralea lachnostachys. Transformed roots grew very rapidly in Gamborg B5 liquid medium with a doubling time of the culture of 38 hours. Whatever the culture conditions, the two furanocoumarins usually found in roots of Psoralea plants, psoralen and angelicin, were not detected in cultured transformed and non transformed roots even when some chitosan was added to the medium. However, 669 μg.g−1 dry matter of psoralen and 215 μg.g−1 dry matter of angelicin were found in roots from soil grown plants. A possible translocation of these compounds from the aerial parts to the roots is suggested. Springer-Verlag, 1992 Agrobacterium rhizogenes nationallicence angelicin nationallicence furanocoumarin nationallicence Psoralea nationallicence psoralen nationallicence transformed root nationallicence B5 medium : Gamborg's medium (Flow laboratories's formulation) nationallicence NAA : Naphthaleneacetic acid nationallicence Nguyen Christophe Laboratoire Agronomie et Environenment, ENSAIA-INRA, 2, Avenue de la Forêt de Haye, 54500, Vandoeuvre-lès-Nancy, France aut Bourgaud Frédéric Laboratoire Agronomie et Environenment, ENSAIA-INRA, 2, Avenue de la Forêt de Haye, 54500, Vandoeuvre-lès-Nancy, France aut Forlot Paul Laboratoires Pharmaceutiques BERGADERM. 46, rue de la Grosse Pierre, Silic 189, 94563, Rungis Cedex, France aut Guckert Armand Laboratoire Agronomie et Environenment, ENSAIA-INRA, 2, Avenue de la Forêt de Haye, 54500, Vandoeuvre-lès-Nancy, France aut Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 424-427 0721-7714 11:8<424 1992 11 299 https://doi.org/10.1007/BF00234375 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00234375 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nguyen Christophe Laboratoire Agronomie et Environenment, ENSAIA-INRA, 2, Avenue de la Forêt de Haye, 54500, Vandoeuvre-lès-Nancy, France aut NATIONALLICENCE 700 1- Bourgaud Frédéric Laboratoire Agronomie et Environenment, ENSAIA-INRA, 2, Avenue de la Forêt de Haye, 54500, Vandoeuvre-lès-Nancy, France aut NATIONALLICENCE 700 1- Forlot Paul Laboratoires Pharmaceutiques BERGADERM. 46, rue de la Grosse Pierre, Silic 189, 94563, Rungis Cedex, France aut NATIONALLICENCE 700 1- Guckert Armand Laboratoire Agronomie et Environenment, ENSAIA-INRA, 2, Avenue de la Forêt de Haye, 54500, Vandoeuvre-lès-Nancy, France aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 424-427 0721-7714 11:8<424 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer