caa a22 4500 469028548 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00234365 doi (NATIONALLICENCE)springer-10.1007/BF00234365 Somatic embryogenesis and plant regeneration from immature embryos of western larch [Elektronische Daten] [R. Thompson, Patrick von Aderkas] Somatic embryogenesis was initiated from immature embryos of western larch (Larix occidentalis Nutt.) on media containing 2,4-dichlorophenoxyacetic acid and N6- benzyladenine. The effects of explant type and ammonium nitrate and glutamine concentrations on initiation were tested. Although 21-93% of explants rendered cultures in various experiments, only 3% yielded sustainable embryogenic lines. Excised embryos at the early cotyledonary stage were optimal for initiation. Maturation of somatic embryos was promoted by abscisic acid. Response to abscisic acid concentrations and duration of exposure to abscisic acid varied with genotype. Maximal results were obtained with 0.025 μ M abscisic acid for 1 to 2 weeks followed by individual culture on medium without growth regulators. Mature somatic embryos developed into shoots with roots. Plantlets have been established in peat. Springer-Verlag, 1992 Thompson R. Centre for Forest Biology, Department of Biology, University of Victoria, V8W 2Y2, Victoria, British Columbia, Canada aut von Aderkas Patrick Centre for Forest Biology, Department of Biology, University of Victoria, V8W 2Y2, Victoria, British Columbia, Canada aut Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 379-385 0721-7714 11:8<379 1992 11 299 https://doi.org/10.1007/BF00234365 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00234365 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thompson R. Centre for Forest Biology, Department of Biology, University of Victoria, V8W 2Y2, Victoria, British Columbia, Canada aut NATIONALLICENCE 700 1- von Aderkas Patrick Centre for Forest Biology, Department of Biology, University of Victoria, V8W 2Y2, Victoria, British Columbia, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 379-385 0721-7714 11:8<379 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer