caa a22 4500 469028556 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00234374 doi (NATIONALLICENCE)springer-10.1007/BF00234374 Microtubule pattern and the occurrence of pre-prophase bands in embryogenic cultures of black spruce ( Pieca mariana Mill.) and non-embryogenic cultures of jack pine ( Pinus banksiana Lamb.) [Elektronische Daten] [T. Tautorus, H. Wang, L. Fowke, D. Dunstan] Summary: The organization of microtubules during interphase and prophase in embryogenic cultures of black spruce (Picea mariana) was investigated by indirect immunofluorescence. Somatic embryos of black spruce possessed an extensively branched and interconnecting network of fine interphase cortical microtubules. The development of pre-prophase bands (PPBs) in embryogenic black spruce cultures was compared with that in non-embryogenic cell cultures of jack pine (Pinus banksiana). PPBs in both species were initially arranged as a very broad array of microtubules, later (early to mid-prophase) becoming narrower and more intensely fluorescent. The occurrence of pre-prophase bands in relation to the number of phragmoplasts (i.e. PPB index) of black spruce somatic embryos was significantly higher (p<0.01) than that found for jack pine cells. Springer-Verlag, 1992 Tautorus T. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada aut Wang H. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada aut Fowke L. Department of Biology, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada aut Dunstan D. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada aut Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 419-423 0721-7714 11:8<419 1992 11 299 https://doi.org/10.1007/BF00234374 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00234374 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tautorus T. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- Wang H. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- Fowke L. Department of Biology, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 700 1- Dunstan D. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 419-423 0721-7714 11:8<419 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer