caa a22 4500 469028572 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00234376 doi (NATIONALLICENCE)springer-10.1007/BF00234376 Resynthesis of Brassica carinata by protoplast fusion and recovery of a novel cytoplasmic hybrid [Elektronische Daten] [S. Narasimhulu, P. Kirti, Shyam Prakash, V. Chopra] Brassica carinata (2n=34, BBCC), was synthesized by fusing dark grown etiolated hypocotyl protoplasts of B. nigra (2n=16, BB) with green mesophyll protoplasts of B. oleracea (2n=18,CC) using polyethylene glycol. Heterokaryons could be microscopically distinguished from the parental types by their dark green chloroplasts in the colourless hypocotyl protoplast background. The mean heterokaryotic fusion frequency estimated on the basis of this morphological distinction was about 16%. A total of 626 calli were obtained, of which 92 regenerated shoots after transfer to zeatin (2 mg/l) supplemented MS medium. Of these, 81 calli differentiated into plants morphologically similar to naturally occurring B. carinata and 11 calli yielded plants resembling parental types. Meiosis in seven hybrid plants showed the chromosome number to be 2n=34 the sum of B. nigra and B. oleracea chromosomes. Molecular confirmation of the amphidiploid nature of hybrids was obtained by probing with a B. juncea derived genomic clone. The use of chloroplast and mitochondrial specific gene probes, revealed that one plant was a cytoplasmic hybrid having cp DNA sequences of both B. oleracea and B. nigra and mt DNA sequences of B. nigra. Springer-Verlag, 1992 Brassica nigra nationallicence B. oleracea nationallicence B. carinata nationallicence Protoplast fusion nationallicence Cytoplasmic hybrid nationallicence PEG : Polyethylene glycol nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence NAA : αNaphthaleneacetic acid nationallicence IBA : Indole-3-butyric acid nationallicence BAP : 6-Benzylaminopurine nationallicence MS : Murashige and Skoog (1962) nationallicence Narasimhulu S. Biotechnology Centre, Indian Agricultural Research Institute, 10012, New Delhi, India aut Kirti P. Biotechnology Centre, Indian Agricultural Research Institute, 10012, New Delhi, India aut Prakash Shyam Biotechnology Centre, Indian Agricultural Research Institute, 10012, New Delhi, India aut Chopra V. Biotechnology Centre, Indian Agricultural Research Institute, 10012, New Delhi, India aut Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 428-432 0721-7714 11:8<428 1992 11 299 https://doi.org/10.1007/BF00234376 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00234376 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Narasimhulu S. Biotechnology Centre, Indian Agricultural Research Institute, 10012, New Delhi, India aut NATIONALLICENCE 700 1- Kirti P. Biotechnology Centre, Indian Agricultural Research Institute, 10012, New Delhi, India aut NATIONALLICENCE 700 1- Prakash Shyam Biotechnology Centre, Indian Agricultural Research Institute, 10012, New Delhi, India aut NATIONALLICENCE 700 1- Chopra V. Biotechnology Centre, Indian Agricultural Research Institute, 10012, New Delhi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 428-432 0721-7714 11:8<428 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer