caa a22 4500 469028599 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00234367 doi (NATIONALLICENCE)springer-10.1007/BF00234367 The effect of temperature on growth, indole alkaloid accumulation and lipid composition of Catharanthus roseus cell suspension cultures [Elektronische Daten] [Leena Toivonen, Simo Laakso, Heikki Rosenqvist] Cell suspension cultures of Catharanthus roseus were used to study the effect of temperature on plant cell lipids and indole alkaloid accumulation. Lowering the cultivation temperature increased the total fatty acid content per cell dry weight relative to that at higher temperatures, mainly because of increased accumulation of unsaturated C18 acids. In addition, an increase in the relative proportion of phosphatidylcholine and phosphatidylethanolamine was observed. Within individual lipids, the degree of unsaturation was increased and the mean fatty acid chain length decreased with reducing temperature. These changes may be interpreted as modifying the cell membrane fluidity to keep it optimal for growth and metabolism at each temperature. In spite of membrane modifications, the indole alkaloid content of the cells or the medium was not affected by temperature change. Springer-Verlag, 1992 Toivonen Leena Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut Laakso Simo Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut Rosenqvist Heikki Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 390-394 0721-7714 11:8<390 1992 11 299 https://doi.org/10.1007/BF00234367 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00234367 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toivonen Leena Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut NATIONALLICENCE 700 1- Laakso Simo Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut NATIONALLICENCE 700 1- Rosenqvist Heikki Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 390-394 0721-7714 11:8<390 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer