caa a22 4500 469028602 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF00234373 doi (NATIONALLICENCE)springer-10.1007/BF00234373 Protoplast culture in high molecular oxygen atmospheres [Elektronische Daten] [E Vaz, I. Slamet, A. Khatun, E. Cocking, J. Power] The effect of a molecular oxygen atmosphere on protoplast culture, compared with normal cultural procedures, was investigated for tomato, rice and jute protoplast systems. Protoplasts, in unsealed Petri dishes were cultured in a sealed 250 ml volume container in which the atmosphere was replaced with sterile molecular oxygen (O2 100% v/v) achieved by a one minute oxygen flow (10 mBar). Controls were cultured in a similar way but with a normal atmosphere (20.5% v/v oxygen). For all three species a major improvement in plating efficiency and colony formation was observed in the oxygen-enriched atmospheres. In the case of rice, cultivar Taipei 309, a significant increase in plant regeneration capacity, from protoplast-derived colonies, was observed for the high oxygen cultures. Springer-Verlag, 1992 Vaz E. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK aut Slamet I. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK aut Khatun A. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK aut Cocking E. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK aut Power J. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK aut Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 416-418 0721-7714 11:8<416 1992 11 299 https://doi.org/10.1007/BF00234373 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00234373 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vaz E. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK aut NATIONALLICENCE 700 1- Slamet I. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK aut NATIONALLICENCE 700 1- Khatun A. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK aut NATIONALLICENCE 700 1- Cocking E. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK aut NATIONALLICENCE 700 1- Power J. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/8(1992-07-01), 416-418 0721-7714 11:8<416 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer