caa a22 4500 469028629 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00235254 doi (NATIONALLICENCE)springer-10.1007/BF00235254 Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium [Elektronische Daten] [Hitoshi Wake, Akinobu Akasaka, Hironori Umetsu, Yoshihiro Ozeki, Koichiro Shimomura, Tadashi Matsunaga] Summary: Somatic embryos of Daucus carota L. developed into plantlets at high frequency after addition of an extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. High molecular weight, nondialyzing fraction, separated from the extract, possessed enhanced plantlet formation promoting activity. Plantlet formation frequency was 60 % after addition of nondialysate (100 mg/l) compared to 28 % without addition. Embryos treated with the nondialysate contained five times more chlorophyll than nontreated embryos after 6 days of culture. The chlorophyll a/b ratio of 4-day old treated somatic embryos was found to be similar to that of zygotic embryos. However, the chlorophyll a/b ratio of plantlets induced from nontreated somatic embryos was variable. Nondialysate was fractionated by ultracentrifugation and an active component obtained, which gave a maximum plantlet formation frequency of 71 %, and induced rapid greening of shoots. Springer-Verlag, 1992 Marine Cyanobacteria nationallicence Daucus carota L nationallicence Plantlet formation nationallicence Chlorophyll content nationallicence MS : Murashige and Skoog medium nationallicence 2,4D : 2,4-dichlorophenoxyacetic acid nationallicence PCV : packed cell volume nationallicence μE : μEinstein nationallicence Chl : Chlorophyll nationallicence vvm : volume of air, volume of medium per minute nationallicence Wake Hitoshi Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut Akasaka Akinobu Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut Umetsu Hironori Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut Ozeki Yoshihiro Department of Biology, University of Tokyo, Komaba, 153, Tokyo, Japan aut Shimomura Koichiro Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, Tsukuba, 305, Ibaraki, Japan aut Matsunaga Tadashi Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 62-65 0721-7714 11:2<62 1992 11 299 https://doi.org/10.1007/BF00235254 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235254 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wake Hitoshi Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut NATIONALLICENCE 700 1- Akasaka Akinobu Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut NATIONALLICENCE 700 1- Umetsu Hironori Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut NATIONALLICENCE 700 1- Ozeki Yoshihiro Department of Biology, University of Tokyo, Komaba, 153, Tokyo, Japan aut NATIONALLICENCE 700 1- Shimomura Koichiro Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Matsunaga Tadashi Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 62-65 0721-7714 11:2<62 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer