caa a22 4500 469028645 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00235257 doi (NATIONALLICENCE)springer-10.1007/BF00235257 Expression of the GUS-gene in the monocot tulip after introduction by particle bombardment and Agrobacterium [Elektronische Daten] [A. Wilmink, B. van de Ven, J. Dons] Gene transfer to the monocotyledon tulip (Tulipa sp. L.) was obtained both by particle bombardment and Agrobacterium transformation. Using a Particle Delivery System, transient expression of the reporter gene for ßglucuronidase was demonstrated. It was shown that the CAMV 35S as well as the TR2' promoter were active in flower stem expiants. Various wildtype and disarmed Agrobacterium strains, harbouring the 35S GUSintron gene on a binary plasmid, were used for infection of flower stem expiants of 7 cultivars and 7 botanical Tulipa species. In nine genotypes the GUSintron gene was expressed, despite the fact that tulip tissue did not produce detectable amounts of virulence-inducing substances. Agrobacterium rhizogenes appeared to be most effective in gene transfer to tulip tissue. Springer-Verlag, 1992 Wilmink A. Department of Developmental Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut van de Ven B. Department of Developmental Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut Dons J. Department of Developmental Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 76-80 0721-7714 11:2<76 1992 11 299 https://doi.org/10.1007/BF00235257 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235257 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wilmink A. Department of Developmental Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- van de Ven B. Department of Developmental Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Dons J. Department of Developmental Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 76-80 0721-7714 11:2<76 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer