caa a22 4500 469028653 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00235263 doi (NATIONALLICENCE)springer-10.1007/BF00235263 Micropropagation of an elite Darjeeling tea clone [Elektronische Daten] [Timirbaran Jha, S. Sen] Shoot cultures of Camellia sinensis (L.) O. Kuntz var. T-78, an elite Darjeeling tea clone, were established from cotyledonary nodes and shoot tips of germinated seedlings as well as from nodal explants of field grown plants. Shoot multiplication rate ranged from 4x in nodal explants to 35x in cotyledonary nodes after 18 weeks of culture. Rooting was achieved in 80-90% micro-shoots by either placing them on an inductive medium for 10 d and then transferring shoots to hormone-free medium, or by treating micro-shoots with a chronic dose of IBA (500 mg/l) for 30-40 min. Rooted plants were established in soil under glasshouse condition at 60% frequency after hardening phase of 4-6 weeks. The regenerated plants show a constant chromosome number of 2n=30 and are morphologically true to type. This procedure can be applied for conservation and utilisation of an elite clone of Darjeeling tea. Springer-Verlag, 1992 Jha Timirbaran Plant Molecular & Cellular Genetics, Bose Institute, P-1/12, C.I.T. Scheme VII-M, 700 054, Calcutta, India aut Sen S. Plant Molecular & Cellular Genetics, Bose Institute, P-1/12, C.I.T. Scheme VII-M, 700 054, Calcutta, India aut Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 101-104 0721-7714 11:2<101 1992 11 299 https://doi.org/10.1007/BF00235263 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235263 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jha Timirbaran Plant Molecular & Cellular Genetics, Bose Institute, P-1/12, C.I.T. Scheme VII-M, 700 054, Calcutta, India aut NATIONALLICENCE 700 1- Sen S. Plant Molecular & Cellular Genetics, Bose Institute, P-1/12, C.I.T. Scheme VII-M, 700 054, Calcutta, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 101-104 0721-7714 11:2<101 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer