caa a22 4500 469028661 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00235255 doi (NATIONALLICENCE)springer-10.1007/BF00235255 Tissue culture of endod ( Phytolacca dodecandra L'Herit): growth and production of ribosome-inactivating proteins [Elektronische Daten] [M. Bonness, T. Mabry] Summary: Leaves and stems from endod (Phytolacca dodecandra L'Herit), known to produce the 29 kDa ribosome-inactivating protein (RIP) dodecandrin, were initiated into tissue culture. Callus and suspension cultures were maintained on modified Murashige and Skoog medium plus 1.0 mg/l 2,4-dichlorophenoxyacetic acid. Six callus and two suspension cell lines were screened for dodecandrin production by western blots with affinitypurified antiserum. Antiribosomal activity of culture extracts was tested by in vitro translation assays. One suspension cell line was found to be free of immunoreactive proteins and a ribosome inhibitor. All other cell lines contain a ribosome inhibitor, although only two callus cell lines show detectable amounts of immunoreactive proteins at the same Mr as dodecandrin. Other immuno-reactive proteins were detected in callus (Mr 31000, 33000, 41000 and 43000) and in suspension cells (Mr 23000 and ∼43000), and may be ribosome inhibitors related to dodecandrin—either other RIPs or dodecandrin at various stages of processing. Springer-Verlag, 1992 Phytolacca dodecandra nationallicence endod nationallicence ribosomeinactivating protein nationallicence dodecandrin nationallicence tissue culture nationallicence Bonness M. Department of Botany, The University of Texas at Austin, 78713, Austin, Texas, USA aut Mabry T. Department of Botany, The University of Texas at Austin, 78713, Austin, Texas, USA aut Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 66-70 0721-7714 11:2<66 1992 11 299 https://doi.org/10.1007/BF00235255 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235255 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bonness M. Department of Botany, The University of Texas at Austin, 78713, Austin, Texas, USA aut NATIONALLICENCE 700 1- Mabry T. Department of Botany, The University of Texas at Austin, 78713, Austin, Texas, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 66-70 0721-7714 11:2<66 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer