caa a22 4500 46902867X CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00235258 doi (NATIONALLICENCE)springer-10.1007/BF00235258 Embryogenic cell suspensions of Triticum aestivum x Leymus angustus F1 hybrids: characterization and plant regeneration [Elektronische Daten] [Zohreh Tabaeizadeh, Nathalie Campeau] Summary: Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they continued rapid growth up to 10 days after subculturing. Flow cytometric studies of the cell cycle over a 7 day culture period showed that the majority of cells were in G1 phase while the rest were either in S or G2. During the 7 days of culture, no significant differences in DNA distribution patterns were observed. The cells from suspension cultures produced somatic embryos when they were transferred to different solid media. The embryos germinated and gave rise to plantlets which were successfully rooted and transferred to soil. Springer-Verlag, 1992 Cell suspension nationallicence Somatic embryogenesis nationallicence Triticum aestivum nationallicence Leymus angustus nationallicence Plant regeneration nationallicence Tabaeizadeh Zohreh Department of Biological Sciences, University of Quebec in Montreal, Station "A”, P.O. Box 8888, H3C 3P8, Montreal, Quebec, Canada aut Campeau Nathalie Department of Biological Sciences, University of Quebec in Montreal, Station "A”, P.O. Box 8888, H3C 3P8, Montreal, Quebec, Canada aut Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 81-85 0721-7714 11:2<81 1992 11 299 https://doi.org/10.1007/BF00235258 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235258 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tabaeizadeh Zohreh Department of Biological Sciences, University of Quebec in Montreal, Station "A”, P.O. Box 8888, H3C 3P8, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Campeau Nathalie Department of Biological Sciences, University of Quebec in Montreal, Station "A”, P.O. Box 8888, H3C 3P8, Montreal, Quebec, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 81-85 0721-7714 11:2<81 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer