caa a22 4500 469028696 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00235252 doi (NATIONALLICENCE)springer-10.1007/BF00235252 Genetic transformation of sweet potato by particle bombardment [Elektronische Daten] [C. Prakash, U. Varadarajan] Transient and stable expression of foreign genes has been achieved in sweet potato using the particle bombardment system of gene delivery. Callus and root isolates of two genotypes (Jewel and TIS-70357) with positive signs of transformation have been recovered. Tungsten microcarriers coated with plasmid DNA (pBI 221 containing the gusA gene) were accelerated at high velocity using a biolistic device into sweet potato target tissues. Histochemical examination of bombarded leaf and petiole explants revealed that most had cells expressing the gusA gene. When explants were cultured, calli and roots developed in most bombarded tissues. Similar results but with a lower frequency of transformation were observed when the plasmid pBI 121 (with gusA and antibiotic resistance npt II genes) was employed and bombarded explants cultured on an antibiotic selection medium. Subcultured roots and calli were positive for gusA expression when tested even after one year of in vitro culture, and thus the expression of the foreign gene is fairly stable. The particle bombardment approach of gene delivery appears to have a potential for generating transgenic sweet potatoes with useful agronomic traits. Springer-Verlag, 1992 pomoea batatas nationallicence Tissue culture nationallicence Gene transfer nationallicence Biolistics nationallicence BA : 6-benzylaminopurine nationallicence CaMV : cauliflower mosaic virus nationallicence 2,4-D : 2, 4-dichlorophenoxyacetic acid nationallicence GUS : ß glucuronidase nationallicence NAA : naphthaleneaceticacid nationallicence nos : nopaline synthase gene nationallicence NPT II : neomycin phosphotransferase II nationallicence MS : Murashige and Skoog (1962) nationallicence MS-CP : MS cell proliferation medium nationallicence Prakash C. Plant Molecular and Cellular Genetics Laboratory, School of Agriculture and Home Economics, Tuskegee University, Milbank Hall, 36088, Tuskegee, AL, USA aut Varadarajan U. Plant Molecular and Cellular Genetics Laboratory, School of Agriculture and Home Economics, Tuskegee University, Milbank Hall, 36088, Tuskegee, AL, USA aut Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 53-57 0721-7714 11:2<53 1992 11 299 https://doi.org/10.1007/BF00235252 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235252 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Prakash C. Plant Molecular and Cellular Genetics Laboratory, School of Agriculture and Home Economics, Tuskegee University, Milbank Hall, 36088, Tuskegee, AL, USA aut NATIONALLICENCE 700 1- Varadarajan U. Plant Molecular and Cellular Genetics Laboratory, School of Agriculture and Home Economics, Tuskegee University, Milbank Hall, 36088, Tuskegee, AL, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 53-57 0721-7714 11:2<53 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer