caa a22 4500 46902870X CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00235253 doi (NATIONALLICENCE)springer-10.1007/BF00235253 Shoot regeneration from spinach hypocotyl segments by short term treatment with 5,6-dichloro-indole-3-acetic acid [Elektronische Daten] [M. Mii, M. Nakano, K. Okuda, M. Iizuka] Factors affecting shoot regeneration from hypocotyl segments of spinach (Spinacia oleracea L.) were investigated. When expiants were cultured on medium containing 10 mg/l IAA for 7 weeks, 3 out of 9 cultivars showed relatively high shoot regeneration response (15 - 35%). The other PGRs tested had no effect on shoot regeneration. However, the transfer of explants from auxin-containing medium to auxin-free medium 20 d after culture induced shoot formation from expiants cultured on media containing each of the auxin sources tested individually. By applying this short term auxin treatment, more than 80% shoot regeneration was obtained on medium containing 5-20 mg/l 5,6-Cl2-IAA, compared to less than 30% with 10-20 mg/l IAA treatment. Springer-Verlag, 1992 Mii M. Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut Nakano M. Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut Okuda K. Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut Iizuka M. Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 58-61 0721-7714 11:2<58 1992 11 299 https://doi.org/10.1007/BF00235253 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235253 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mii M. Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut NATIONALLICENCE 700 1- Nakano M. Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut NATIONALLICENCE 700 1- Okuda K. Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut NATIONALLICENCE 700 1- Iizuka M. Faculty of Horticulture, Chiba University, 648 Matsudo, 271, Chiba, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 58-61 0721-7714 11:2<58 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer