caa a22 4500 469028718 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00235256 doi (NATIONALLICENCE)springer-10.1007/BF00235256 Somatic embryogenesis and plant regeneration from leaflets of peanut, Arachis hypogaea [Elektronische Daten] [Charleen Baker, Hazel Wetzstein] Somatic embryos were induced on peanut (Arachis hypogaea) leaflets from aseptically germinated embryo axes. Leaflet size influenced percent somatic embryogenesis; 5-8 mm long cut leaflets were superior to 2-3 mm long uncut leaflets. Maximum embryogenesis of 14.6% was obtained after a 15 d incubation on induction medium (modified MS with B5 vitamins, 30 g/l sucrose, 4 g/l Gel-Gro, 40 mg/l 2,4-D +0.2 mg/l kinetin) followed by transfer to a secondary medium with 5 mg/l 2,4-D+0.2 mg/l kinetin. Primary somatic embryos were fused along the axes with no distinct cotyledons, but secondary embryos had single axes with two cotyledons. Other treatments had lower percent embryogenesis, no secondary embryogenesis, and embryos with single axes with two cotyledons. Some somatic embryos converted into normal plants capable of greenhouse survival. Springer-Verlag, 1992 Baker Charleen Horticulture Department, University of Georgia, 1111 Plant Science Building, 30602, Athens, GA, USA aut Wetzstein Hazel Horticulture Department, University of Georgia, 1111 Plant Science Building, 30602, Athens, GA, USA aut Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 71-75 0721-7714 11:2<71 1992 11 299 https://doi.org/10.1007/BF00235256 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235256 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baker Charleen Horticulture Department, University of Georgia, 1111 Plant Science Building, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Wetzstein Hazel Horticulture Department, University of Georgia, 1111 Plant Science Building, 30602, Athens, GA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 71-75 0721-7714 11:2<71 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer