caa a22 4500 469028726 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00235260 doi (NATIONALLICENCE)springer-10.1007/BF00235260 Production and characterization of intergeneric somatic hybrids of Trachystoma ballii and Brassica juncea [Elektronische Daten] [P. Kirti, S. Narasimhulu, Shyam Prakash, V. Chopra] Intergeneric somatic hybrids, Trachystoma ballii (2n=16)+B. juncea (2n=38), were obtained by fusing mesophyll protoplasts of T. ballii and hypocotyl protoplasts of B. juncea using polyethylene glycol. The heterokaryotic fusion frequency was around 23%. Plants were regenerated from 10 out of 2×104 calli of which four were hybrids. Hybrids were intermediate between the parents in general morphology. However, in some characters one of the parents dominated. All the plants were symmetric in their chromosomal constitution as revealed by the formation of 26 bivalents at metaphase-I of meiosis. Two trivalents and 2 univalents were also observed in some pollen mother cells. Hybrid nature was also confirmed by ‘Southern' hybridization of DNA of one regenerated plant restricted with Hind III and probed with the nick translated plasmid pTA71 carrying a wheat nuclear r-DNA sequence. Hybrid plant RT 1 showed bands characteristic of both parents. All the plants were absolutely pollen sterile. However, on backcrosses to B. juncea seeds were obtained. Springer-Verlag, 1992 Trachystoma ballii nationallicence Brassica juncea nationallicence Intergeneric somatic hybrid nationallicence Protoplast fusion nationallicence Hybrid characterization nationallicence Kirti P. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Narasimhulu S. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Prakash Shyam Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Chopra V. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 90-92 0721-7714 11:2<90 1992 11 299 https://doi.org/10.1007/BF00235260 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235260 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kirti P. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Narasimhulu S. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Prakash Shyam Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Chopra V. Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 90-92 0721-7714 11:2<90 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer