caa a22 4500 469028734 CHVBK 20180323132737.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00235264 doi (NATIONALLICENCE)springer-10.1007/BF00235264 Transient gene expression in strawberry ( Fragaria x ananassa Duch .) protoplasts and the recovery of transgenic plants [Elektronische Daten] [Marie Nyman, Anita Wallin] Summary: A transient ß-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addition of carrier DNA to the electroporation mixture. Callus selected on 10 μg/ml hygromycin produced shoots which exhibited GUS-activity. The transformed nature of the shoots obtained after selection was confirmed by DNA-analysis. Springer-Verlag, 1992 Nyman Marie Department of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden aut Wallin Anita Department of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden aut Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 105-108 0721-7714 11:2<105 1992 11 299 https://doi.org/10.1007/BF00235264 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00235264 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nyman Marie Department of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden aut NATIONALLICENCE 700 1- Wallin Anita Department of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/2(1992-03-01), 105-108 0721-7714 11:2<105 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer