caa a22 4500 469028750 CHVBK 20180323132738.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00233089 doi (NATIONALLICENCE)springer-10.1007/BF00233089 In vitro culture of coconut endosperm: callus induction and its fatty acids [Elektronische Daten] [Myrna Ceniza, Shinta Ueda, Yukio Sugimura] Summary: Successful induction of callus from coconut endosperm was achieved by using the tissue situated near the micropylar end of a young fruit. For initiation of callus, a high concentration of auxin (20 to 100 ppm) was added to the basal medium containing activated charcoal. Subcultured callus showed a 40-fold increase during culture of three months. Based on the analysis of fatty acid composition, the maturation of endosperm was characterized by an increase in short chain fatty acids (C8, C10, C12, C14)and a decrease in long chain fatty acids (C16, C18: 1, C18: 2). In developing endosperms, proportion of short chain fatty acids was higher in lipids of the antipodal than those of other regions. In the final stage of maturation, around 82% of total fatty acids was short chain fatty acids, while the proportion of long chain fatty acids decreased up to 16%. The fatty acid composition of callus subcultured for six months was comparable to that of the immature endosperm. Lipids were accumulated in callus as globular bodies. Springer-Verlag, 1992 Callus nationallicence Coconut nationallicence Endosperm nationallicence Fatty acid nationallicence Ceniza Myrna Research and Development Section, Pilipinas Kao, Inc., P.O. Box 15, Cagayan de Oro City, Philippines aut Ueda Shinta Research and Development Section, Pilipinas Kao, Inc., P.O. Box 15, Cagayan de Oro City, Philippines aut Sugimura Yukio Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, 321-34, Tochigi, Japan aut Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 546-549 0721-7714 11:11<546 1992 11 299 https://doi.org/10.1007/BF00233089 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233089 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ceniza Myrna Research and Development Section, Pilipinas Kao, Inc., P.O. Box 15, Cagayan de Oro City, Philippines aut NATIONALLICENCE 700 1- Ueda Shinta Research and Development Section, Pilipinas Kao, Inc., P.O. Box 15, Cagayan de Oro City, Philippines aut NATIONALLICENCE 700 1- Sugimura Yukio Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikaimachi, Haga, 321-34, Tochigi, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 546-549 0721-7714 11:11<546 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer