caa a22 4500 469028785 CHVBK 20180323132738.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00233098 doi (NATIONALLICENCE)springer-10.1007/BF00233098 Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells [Elektronische Daten] [Jun Cao, Xiaolan Duan, David McEIroy, Ray Wu] Summary: Suspension cells of Oryza sativa L. (rice) were transformed, by microprojectile bombardment, with plasmids carrying the coding region of the Streptomyces hygroscopicus phosphinothricin acetyl transferase (PAT) gene (bar) under the control of either the 5′ region of the rice actin 1 gene (Act1) or the cauliflower mosaic virus (CaMV) 35S promoter. Subsequently regenerated plants display detectable PAT activity and are resistant to BASTATM, a phosphinothricin (PPT)-based herbicide. DNA gel blot analyses showed that PPT resistant rice plants contain a bar-hybridizing restriction fragment of the expected size. This report shows that expression of the bar gene in transgenic rice plants confers resistance to PPT-based herbicide by suppressing an increase of ammonia in plants after spraying with the herbicide. Springer-Verlag, 1992 Transgenic rice nationallicence Herbicide resistance nationallicence Phosphinothricin acetyl transferase gene nationallicence Cao Jun Plant Science Center, Cornell University, 14853, Ithaca, NY, USA aut Duan Xiaolan Department of Biochemistry, Molecular and Cell Biology, Cornell University, 14853, Ithaca, NY, USA aut McEIroy David Field of Botany, Cornell University, 14853, Ithaca, NY, USA aut Wu Ray Field of Botany, Cornell University, 14853, Ithaca, NY, USA aut Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 586-591 0721-7714 11:11<586 1992 11 299 https://doi.org/10.1007/BF00233098 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233098 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cao Jun Plant Science Center, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Duan Xiaolan Department of Biochemistry, Molecular and Cell Biology, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- McEIroy David Field of Botany, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Wu Ray Field of Botany, Cornell University, 14853, Ithaca, NY, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 586-591 0721-7714 11:11<586 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer