caa a22 4500 469028807 CHVBK 20180323132738.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00233096 doi (NATIONALLICENCE)springer-10.1007/BF00233096 Genotype-specific heat shock proteins in two maize inbreds [Elektronische Daten] [Julie Jorgensen, Jian Weng, Tuan-hua Ho, Henry Nguyen] Summary: Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indicated that Mol7 possesses greater potential than B73 to acquire thermal tolerance. Poly(A)+ RNA, extracted from leaf blades, was translated in vitro in the presence of 35S-methionine and the translation products separated by twodimensional gel electrophoresis. Major genotypic differences were observed in the translation products. Mo 17 synthesized twelve unique heat shock proteins in the 15-18 kD range, but B73 synthesized only three unique heat shock proteins in the same range. DNA polymorphisms were observed between the maize lines using 32P labeled heat shock protein gene probes. Springer-Verlag, 1992 Jorgensen Julie Plant Genetics Laboratory, Mail Stop 2122, Texas Tech University, 79409, Lubbock, TX, USA aut Weng Jian Plant Genetics Laboratory, Mail Stop 2122, Texas Tech University, 79409, Lubbock, TX, USA aut Ho Tuan-hua Department of Biology, Washington University, 63130, St. Louis, MO, USA aut Nguyen Henry Plant Genetics Laboratory, Mail Stop 2122, Texas Tech University, 79409, Lubbock, TX, USA aut Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 576-580 0721-7714 11:11<576 1992 11 299 https://doi.org/10.1007/BF00233096 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233096 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jorgensen Julie Plant Genetics Laboratory, Mail Stop 2122, Texas Tech University, 79409, Lubbock, TX, USA aut NATIONALLICENCE 700 1- Weng Jian Plant Genetics Laboratory, Mail Stop 2122, Texas Tech University, 79409, Lubbock, TX, USA aut NATIONALLICENCE 700 1- Ho Tuan-hua Department of Biology, Washington University, 63130, St. Louis, MO, USA aut NATIONALLICENCE 700 1- Nguyen Henry Plant Genetics Laboratory, Mail Stop 2122, Texas Tech University, 79409, Lubbock, TX, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 576-580 0721-7714 11:11<576 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer