caa a22 4500 469028823 CHVBK 20180323132738.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00233091 doi (NATIONALLICENCE)springer-10.1007/BF00233091 Differentiation of somatic embryos from protoplasts isolated from embryogenic suspension cultures of Abies alba L [Elektronische Daten] [Sabine Hartmann, Hermann Lang, Gerhard Reuther] Embryogenic suspension cultures of Abies alba were established using an embryogenic suspensor mass culture originating from the zygotic embryo in immature seed explants (Schuller et al. 1989). Protoplasts were isolated from the suspension material. The protoplasts were immobilized in alginate layers in order to follow the development of single protoplasts. During the first days of protoplast culture a modified Kao and Michayluk (1975) medium proved to be necessary for subsequent divisions. The formation of proembryos succeeded within 2-3 weeks when subcultured with a modified Schenk and Hildebrandt (1972) liquid medium. Light, enhanced sugar concentration, and the addition of abscisic acid led to the formation of slightly green "torpedo”-shaped somatic embryos after 6-8 weeks from protoplast isolation. Springer-Verlag, 1992 Hartmann Sabine Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstraße 70, W-6000, Frankfurt am Main, Federal Republic of Germany aut Lang Hermann Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstraße 70, W-6000, Frankfurt am Main, Federal Republic of Germany aut Reuther Gerhard Institut für Biologie, Fachgebiet Botanik, Forschungsanstalt Geisenheim, von-Lade-Straße 1, W-6222, Geisenheim am Rhein, Federal Republic of Germany aut Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 554-557 0721-7714 11:11<554 1992 11 299 https://doi.org/10.1007/BF00233091 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233091 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hartmann Sabine Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstraße 70, W-6000, Frankfurt am Main, Federal Republic of Germany aut NATIONALLICENCE 700 1- Lang Hermann Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstraße 70, W-6000, Frankfurt am Main, Federal Republic of Germany aut NATIONALLICENCE 700 1- Reuther Gerhard Institut für Biologie, Fachgebiet Botanik, Forschungsanstalt Geisenheim, von-Lade-Straße 1, W-6222, Geisenheim am Rhein, Federal Republic of Germany aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 554-557 0721-7714 11:11<554 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer