caa a22 4500 469028858 CHVBK 20180323132738.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00233094 doi (NATIONALLICENCE)springer-10.1007/BF00233094 Electroporation and PEG delivery of DNA into maize microspores [Elektronische Daten] [Anne Fennell, Randal Hauptmann] Summary: The ability to deliver and detect reporter gene activity in maize microspores was tested. Tested expression vectors contained the chloramphenicol acetyl transferase (CAT) gene and one of the following promoter-intron combinations: 1) cauliflower mosaic virus (CaMV 35S), 2) CaMV 35S + maize alcohol dehydrogenase 1 intron 6 (Adh1-I6), 3) maize alcohol dehydrogenase 1 + intron 1 (Adh1-I1), or 4) maize ubiquitin 1 + intron 1 (Ubiq 1-I1) promoter + intron. The expression vectors were delivered into maize microspores using electroporation or polyethylene glycol (PEG). Both methods were effective for delivering free DNA into microspores. Although all four promoters were active in maize protoplasts, only two promoters were active in maize microspores. The CaMV 35S and the Adh1 promoters did not promote gene expression in maize microspore. The CaMV 35S + Adh1-I6 and Ubiq1-I1 promoters produced high levels of CAT activity in maize microspores. Springer-Verlag, 1992 Microspore nationallicence Electroporation nationallicence Transformation nationallicence Zea mays nationallicence Fennell Anne Plant Molecular Biology Center, Northern Illinois University, 60115, DeKalb, IL, USA aut Hauptmann Randal Plant Molecular Biology Center, Northern Illinois University, 60115, DeKalb, IL, USA aut Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 567-570 0721-7714 11:11<567 1992 11 299 https://doi.org/10.1007/BF00233094 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233094 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fennell Anne Plant Molecular Biology Center, Northern Illinois University, 60115, DeKalb, IL, USA aut NATIONALLICENCE 700 1- Hauptmann Randal Plant Molecular Biology Center, Northern Illinois University, 60115, DeKalb, IL, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 567-570 0721-7714 11:11<567 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer