caa a22 4500 469028866 CHVBK 20180323132738.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00233088 doi (NATIONALLICENCE)springer-10.1007/BF00233088 Palmer C. Department of Plant Science, University of Manitoba, R3T 2N2, Winnipeg, MB, Canada aut Enhanced shoot regeneration from Brassica campestris by silver nitrate [Elektronische Daten] [C. Palmer] Summary: The morphogenetic response of Brassica campestris genotype R500 to inhibitors of ethylene biosynthesis and action was investigated. A medium containing 1.0 mg.l−1 NAA, 2.0 mg.l−1 BAP, and 30 or 60 μM AgNO3 significantly enhanced both the percentage shoot regeneration and the number of shoots per cotyledon expiant. Although callus proliferation occurred on hypocotyl segments, no shoots were formed in response to AgNO3 with expiants older than five days. Cotyledons older than six days formed shoots only with AgNO3. Cobalt chloride at 20 and 30 μM increased cotyledon shoot regeneration but was inferior to AgNO3. Hypocotyl segments were unresponsive. Salicylic acid at 25 and 50 μM prevented both shoot regeneration and callusing without any obvious toxic effects. Removal of expiants from AgNO3 after 12 days did not alter the percentage of shoot regeneration but increased the number of shoots per expiant. This response was dependent on the level of BAP. Percentage shoot regeneration and number of shoots per cotyledon explant were not affected by removal of CoCl2. These results indicate that the poor regenerative capacity of this genotype may be related to ethylene biosynthesis or metabolism. Springer-Verlag, 1992 Brassica campestris nationallicence Shoot Regeneration nationallicence Ethylene nationallicence Inhibitors nationallicence NAA : Naphthalene Acetic Acid nationallicence BAP : 6-Benzylamino Purine nationallicence MS : Murashige and Skoog Medium nationallicence Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 541-545 0721-7714 11:11<541 1992 11 299 https://doi.org/10.1007/BF00233088 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00233088 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Palmer C. Department of Plant Science, University of Manitoba, R3T 2N2, Winnipeg, MB, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 11/11(1992-10-01), 541-545 0721-7714 11:11<541 1992 11 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer