caa a22 4500 469030119 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF01043282 doi (NATIONALLICENCE)springer-10.1007/BF01043282 Increased expression of highly branched N -linked oligosaccharides terminating in N -acetylglucosamine residues in neoplastic and sclerodermal chicken fibroblasts [Elektronische Daten] [Boris Chechik, Bernard Fernandes] Summary: Although neoplastic cells often show a shift towards the expression of largerN-linked oligosaccharides compared to their normal counterparts, little consideration has been given to the possibility that these changes might be a more general phenomenon characteristic of certain neoplastic and non-neoplastic proliferative disorders. TerminalN-acetylglucosamine (GlcNAc) cluster antigen (TGCA) is an immunoreactive epitope(s) of highly branchedN-linked oligosaccharides terminating in GlcNAc residues. Here we have compared the expression of this antigen in normal, neoplastic and sclerodermal chicken fibroblasts by immunomorphological methods. TGCA was detectable in only a few, if any, fibroblasts of normal chicken skin or those cultured from chicken embryos. In contrast, the antigen appeared in 15 to 30% of chicken embryo fibroblasts transformed with avian sarcoma viruses and about 50% of neoplastic fibroblasts of both Rous sarcoma virus-induced fibrosarcomas and carcinogen-induced transplantable fibrosarcomas. Significantly, TGCA was also found in most activated fibroblasts in the skin of chickens with hereditary scleroderma. These results indicate that increased expression of highly branchedN-linked oligosaccharides terminating in GlcNAc residues is characteristic of both neoplastic and sclerodermal chicken fibroblasts. Investigation of this phenomenon may thus provide insight into biochemical pathways involved in neoplastic transformation and pathogenesis of a number of non-neoplastic proliferative connective tissue disorders such as scleroderma. Moreover, changes in the expression of TGCA-positive oligosaccharides (or their modified biochemical counterparts in mammalian species) may have considerable value for diagnosis of several connective tissue diseases. Chapman & Hall, 1992 Chechik Boris The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, M5G 1X5, Toronto, Ontario, Canada aut Fernandes Bernard Department of Pathology, Mount Sinai Hospital, M5G 1X5, Toronto, Ontario, Canada aut The Histochemical Journal Kluwer Academic Publishers 24/1(1992-01-01), 15-20 0018-2214 24:1<15 1992 24 10735 https://doi.org/10.1007/BF01043282 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01043282 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chechik Boris The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, M5G 1X5, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- Fernandes Bernard Department of Pathology, Mount Sinai Hospital, M5G 1X5, Toronto, Ontario, Canada aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/1(1992-01-01), 15-20 0018-2214 24:1<15 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer