caa a22 4500 469030186 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF01043283 doi (NATIONALLICENCE)springer-10.1007/BF01043283 Staining of hyaluronan in rat cerebellum with a hyaluronectin-antihyaluronectin immune complex [Elektronische Daten] [N. Girard, M. Courel, A. Delpech, G. Bruckner, B. Delpech] Summary: The presence of hyaluronan was studied histochemically in the adult rat cerebellum. We used the hyaluronectin-antihyaluronectin immune complex technique based on the high affinity of hyaluronectin for hyaluronan. The immune complex was prepared with hyaluronectin from a human brain extract and an anti-hyaluronectin monoclonal antibody, which does not react with rat hyaluronectin. This is a specific probe for detecting hyaluronan in rat tissues without any reaction for tissue hyaluronectin. Hyaluronan was found at the nodes of Ranvier, in the perineuronal microenvironment of the deep nuclei and at the Purkinje cells surrounding the initial segment of the axon. It was located at the same places as hyaluronectin, in areas specialized in ion exchanges and neurotransmission. This suggests that the hyaluronectin-hyaluronan complex could be involved in these processes. The immune complex technique with anti-hyaluronectin monoclonal antibody thus seems to be a specific and valuable tool for investigations of the distribution of hyaluronan in the rat cerebellum. Chapman & Hall, 1992 Girard N. Laboratory of Molecular Oncology, Centre Henri-Becquerel, rue d'Amiens, F-76000, Rouen, France aut Courel M. Laboratory of Molecular Oncology, Centre Henri-Becquerel, rue d'Amiens, F-76000, Rouen, France aut Delpech A. Laboratory of Histoembryology, Faculté de Médecine Pharmacie, 76800, Saint-Etienne du Rouvray, France aut Bruckner G. Paul Flechsig Institute for Brain Research, University of Leipzig, 7039, Leipzig, Germany aut Delpech B. Laboratory of Molecular Oncology, Centre Henri-Becquerel, rue d'Amiens, F-76000, Rouen, France aut The Histochemical Journal Kluwer Academic Publishers 24/1(1992-01-01), 21-24 0018-2214 24:1<21 1992 24 10735 https://doi.org/10.1007/BF01043283 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01043283 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Girard N. Laboratory of Molecular Oncology, Centre Henri-Becquerel, rue d'Amiens, F-76000, Rouen, France aut NATIONALLICENCE 700 1- Courel M. Laboratory of Molecular Oncology, Centre Henri-Becquerel, rue d'Amiens, F-76000, Rouen, France aut NATIONALLICENCE 700 1- Delpech A. Laboratory of Histoembryology, Faculté de Médecine Pharmacie, 76800, Saint-Etienne du Rouvray, France aut NATIONALLICENCE 700 1- Bruckner G. Paul Flechsig Institute for Brain Research, University of Leipzig, 7039, Leipzig, Germany aut NATIONALLICENCE 700 1- Delpech B. Laboratory of Molecular Oncology, Centre Henri-Becquerel, rue d'Amiens, F-76000, Rouen, France aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/1(1992-01-01), 21-24 0018-2214 24:1<21 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer