caa a22 4500 469030194 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF01046169 doi (NATIONALLICENCE)springer-10.1007/BF01046169 Enzymic potential for fructose 6-phosphate phosphorylation by guard cells and by palisade cells in leaves of the broad bean Vicia faba L [Elektronische Daten] [Daniel Hite, Monique Bodson, William Outlaw Jr] Summary: Guard cells and palisade cells were dissected from freeze-dried leaflets of the broad bean,Vicia faba L. Individual cell samples (6-12 ng) were assayed for ATP-dependent and pyrophosphate-dependent phosphofructokinases. The assay indicator, NADH loss, was monitored in real time in oil droplets with a computer-driven microfluorometer. On a protein basis, both activities were 10-fold higher in guard cells than in palisade cells, indicating (i) elevated carbon metabolism in guard cells to meet demands for energy and carbon skeletons required during stomatal opening, and (ii) parallel glycolytic pathways in guard cells, one responsive to the potent regulatory metabolite fructose 2,6-bisphosphate and the other not. Future work will be devoted to clarifying the roles of the cytosolic and chloroplastic compartments in guard cells. Chapman & Hall, 1992 Hite Daniel Department of Biological Science (B-157), Florida State University, 32306-3050, Tallahassee, FL, USA aut Bodson Monique Centre de Physiologie Végétale Appliquée (IRSIA), Departement de Botanique, Université de Liège, Bât. B-22, Sart Tilman, B-4000, Liège, Belgium aut Outlaw Jr William Department of Biological Science (B-157), Florida State University, 32306-3050, Tallahassee, FL, USA aut The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 368-374 0018-2214 24:6<368 1992 24 10735 https://doi.org/10.1007/BF01046169 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046169 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hite Daniel Department of Biological Science (B-157), Florida State University, 32306-3050, Tallahassee, FL, USA aut NATIONALLICENCE 700 1- Bodson Monique Centre de Physiologie Végétale Appliquée (IRSIA), Departement de Botanique, Université de Liège, Bât. B-22, Sart Tilman, B-4000, Liège, Belgium aut NATIONALLICENCE 700 1- Outlaw Jr William Department of Biological Science (B-157), Florida State University, 32306-3050, Tallahassee, FL, USA aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 368-374 0018-2214 24:6<368 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer