caa a22 4500 469030216 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF01046163 doi (NATIONALLICENCE)springer-10.1007/BF01046163 Histochemical analysis of rat testicular glycoconjugates. 1. Subsets of N-linked saccharides in seminiferous tubules [Elektronische Daten] [Carolyn Jones, Christopher Morrison, Robert Stoddart] Summary: The distribution of N-linked glycans in rat testis has been probed using a panel of lectins derived fromGalanthus nivalis (snowdrop, GNA),Canavalia ensiformis (jack bean, Con A),Lens culinaris (lentil, LCA),Pisum sativum (garden pea, PSA) andPhaseolus vulgaris, erythro- and leucoagglutinins (kidney bean, ePHA and IPHA). Several classes of N-linked glycan were identified in the spermatogenic series, and during differentiation into spermatozoa they altered in both their pattern of distribution and relative abundance. A population of tetra-antennary, non-bisected, complex glycans, detected by IPHA, was lost during the transition from spermatogonia to spermatocytes, while high-mannose structures were acquired; these were most abundant in spermatocytes, as were bi- and tri-antennary complex, non-bisected glycans, the latter becoming increasingly abundant on acrosomes and spermatozoa. Their bisected counterparts were more generally expressed throughout spermatogenic cells, although marked localization onto acrosomes and nuclear caps was again seen. Transition from spermatocytes to spermatids involved mainly changes of the acrosomal granule and nuclear cap, which were carried through to the final stages of differentiation. Sertoli cell surfaces and cytoplasmic granules showed a high level of N-glycan expression. Chapman & Hall, 1992 Jones Carolyn Department of Pathological Sciences, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut Morrison Christopher Department of Immunology, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut Stoddart Robert Department of Pathological Sciences, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 319-326 0018-2214 24:6<319 1992 24 10735 https://doi.org/10.1007/BF01046163 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046163 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jones Carolyn Department of Pathological Sciences, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut NATIONALLICENCE 700 1- Morrison Christopher Department of Immunology, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut NATIONALLICENCE 700 1- Stoddart Robert Department of Pathological Sciences, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 319-326 0018-2214 24:6<319 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 469030216 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer