caa a22 4500 469030232 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF01046170 doi (NATIONALLICENCE)springer-10.1007/BF01046170 Structural variations of different oral basement membranes revealed by cationic dyes and detergent added to aldehyde fixative solution [Elektronische Daten] [H. Chardin, J. Gokani, D. Septier, J. Ruch, M. Goldberg] Summary: The ultrastructural appearance of different types of basement membrane was studied using histochemical methods for visualizing glycosaminoglycans. Samples of rat gingiva and mouse molar germ tissue were fixed either with glutaraldehyde, glutaraldehyde-ruthenium hexammine trichloride (RHT), glutaraldehyde-Cuprolinic Blue (CB) or cetylpyridinium chloride-glutaraldehyde (CPC). Ultrathin sections were stained with uranyl acetate and lead citrate. The results showed that the conventional trilaminar structure of the basement membrane was observed after glutaraldehyde and CB fixation. In contrast, after CPC or RHT fixation, the appearance of the basement membrane was homogeneous without any evidence of a lamina lucida. Furthermore, after single fixation with CPC, the ultrastructure of different basement membranes from oral tissues showed some differences in appearance which were related to their localizations, functions, or both. Chapman & Hall, 1992 Chardin H. Faculté de Chirurgie Dentaire, Biologie et biominéralisation des matrices extracellulaires, 1 rue Maurice Arnoux, 92120, Montrouge, France aut Gokani J. Faculté de Chirurgie Dentaire, Biologie et biominéralisation des matrices extracellulaires, 1 rue Maurice Arnoux, 92120, Montrouge, France aut Septier D. Faculté de Chirurgie Dentaire, Biologie et biominéralisation des matrices extracellulaires, 1 rue Maurice Arnoux, 92120, Montrouge, France aut Ruch J. Institut de Biologie médicale, 11 rue Humann, 67000, Strasbourg, France aut Goldberg M. Faculté de Chirurgie Dentaire, Biologie et biominéralisation des matrices extracellulaires, 1 rue Maurice Arnoux, 92120, Montrouge, France aut The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 375-382 0018-2214 24:6<375 1992 24 10735 https://doi.org/10.1007/BF01046170 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046170 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chardin H. Faculté de Chirurgie Dentaire, Biologie et biominéralisation des matrices extracellulaires, 1 rue Maurice Arnoux, 92120, Montrouge, France aut NATIONALLICENCE 700 1- Gokani J. Faculté de Chirurgie Dentaire, Biologie et biominéralisation des matrices extracellulaires, 1 rue Maurice Arnoux, 92120, Montrouge, France aut NATIONALLICENCE 700 1- Septier D. Faculté de Chirurgie Dentaire, Biologie et biominéralisation des matrices extracellulaires, 1 rue Maurice Arnoux, 92120, Montrouge, France aut NATIONALLICENCE 700 1- Ruch J. Institut de Biologie médicale, 11 rue Humann, 67000, Strasbourg, France aut NATIONALLICENCE 700 1- Goldberg M. Faculté de Chirurgie Dentaire, Biologie et biominéralisation des matrices extracellulaires, 1 rue Maurice Arnoux, 92120, Montrouge, France aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 375-382 0018-2214 24:6<375 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer