caa a22 4500 469030240 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF01046167 doi (NATIONALLICENCE)springer-10.1007/BF01046167 Changes in lectin binding patterns of Leydig cells during fetal and postnatal development in mice [Elektronische Daten] [X. Zhou, H. Kawakami, H. Hirano] Summary: Changes in the lectin binding of mouse Leydig cells during fetal and postnatal development were examined by light- and electron-microscopy using eight different biotinylated lectins (ConA, WGA, RCA-I, UEA-I, GS-I, PNA, SBA and GS-II). At the light-microscopic level, ConA, WGA, RCA-I, UEA-I and GS-I showed the same binding pattern in which all five lectins bound to the plasma membrane and cytoplasm of Leydig cells from the 13th day post coitum (p.c.) to the 8th postnatal week. PNA, SBA and GS-II reactions were positive in the plasma membrane and cytoplasm of Leydig cells from the 13th day p.c. to 15th day post partum (p.p.) but disappeared completely by day 20. At the electron-microscopic level, gold particles representing the GS-I or GS-II binding sites were distributed primarily along the cell surface membrane, including that of microvilli, as well as in the cytoplasm. These results indicate that certain glycoconjugates bearingD-galactose,N-acetyl-D-galactosamine, andN-acetyl-D-glucosamine residues are expressed on the cell surface and in the cytoplasm of Leydig cells during the period from the 13th day p.c. to around the 20th day p.p. The results suggest that these glycoconjugates might play some role in modulating hormone-receptor interaction in the Leydig cells before the 20th day. Furthermore, these results may indicate that sugar residues expressed on the cell surface and in the cytoplasm of Leydig cells are different from those in the fetal-neonatal and adult phases. Chapman & Hall, 1992 Zhou X. Department of Anatomy, Kyorin University School of Medicine, 181, Mitaka, Tokyo, Japan aut Kawakami H. Department of Anatomy, Kyorin University School of Medicine, 181, Mitaka, Tokyo, Japan aut Hirano H. Department of Anatomy, Kyorin University School of Medicine, 181, Mitaka, Tokyo, Japan aut The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 354-360 0018-2214 24:6<354 1992 24 10735 https://doi.org/10.1007/BF01046167 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046167 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou X. Department of Anatomy, Kyorin University School of Medicine, 181, Mitaka, Tokyo, Japan aut NATIONALLICENCE 700 1- Kawakami H. Department of Anatomy, Kyorin University School of Medicine, 181, Mitaka, Tokyo, Japan aut NATIONALLICENCE 700 1- Hirano H. Department of Anatomy, Kyorin University School of Medicine, 181, Mitaka, Tokyo, Japan aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 354-360 0018-2214 24:6<354 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer