caa a22 4500 469030267 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF01046171 doi (NATIONALLICENCE)springer-10.1007/BF01046171 Immunocytochemical study of dystrophin in cultured mouse muscle cells by the quick-freezing and deep-etching method [Elektronische Daten] [Y. Park-Matsumoto, S. Ohno, T. Baba, T. Kobayashi, H. Tsukagoshi] Summary: Dystrophin, the protein product of the Duchenne muscular dystrophy (DMD) gene, is deficient in patients with DMD and in mdx mice. It is immunocytochemically localized in skeletal muscle sarcolemma. However, little is known about the three-dimensional ultrastructural localization of dystrophin and its relationship with other cytoskeletal proteins. We found that dystrophin is localized irregularly, just underneath the plasma membrane in normal cultured mouse myotubes, by using the quick-freezing and deep-etching (QF-DE) method; it was found to be closely linked to actin-like filaments (8-10 nm in diameter), most of which were decorated with myosin subfragment 1, and was attached to the cytoplasmic side of the plasma membrane. These results suggest that dystrophin might play an important role in the preservation of cell membrane stability by connecting actin cytoskeletons with the cytoplasmic side of the plasma membrane. Chapman & Hall, 1992 Park-Matsumoto Y. Department of Neurology, Tokyo Medical and Dental University School of Medicine, 113, Bunkyo-ku, Tokyo, Japan aut Ohno S. Department of Anatomy, Shinshu University School of Medicine, 390, Matsumoto, Japan aut Baba T. Department of Pathology, Shinshu University School of Medicine, 390, Matsumoto, Japan aut Kobayashi T. Department of Neurology, Tokyo Medical and Dental University School of Medicine, 113, Bunkyo-ku, Tokyo, Japan aut Tsukagoshi H. Department of Neurology, Tokyo Medical and Dental University School of Medicine, 113, Bunkyo-ku, Tokyo, Japan aut The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 383-392 0018-2214 24:6<383 1992 24 10735 https://doi.org/10.1007/BF01046171 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046171 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park-Matsumoto Y. Department of Neurology, Tokyo Medical and Dental University School of Medicine, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Ohno S. Department of Anatomy, Shinshu University School of Medicine, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Baba T. Department of Pathology, Shinshu University School of Medicine, 390, Matsumoto, Japan aut NATIONALLICENCE 700 1- Kobayashi T. Department of Neurology, Tokyo Medical and Dental University School of Medicine, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Tsukagoshi H. Department of Neurology, Tokyo Medical and Dental University School of Medicine, 113, Bunkyo-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 383-392 0018-2214 24:6<383 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer