caa a22 4500 469030275 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF01046161 doi (NATIONALLICENCE)springer-10.1007/BF01046161 Simultaneous quantification of DNA and RNA in tissue sections. A comparative analysis of the Methyl Green-Pyronin technique with the Gallocyanin chromalum and Feulgen procedures using image cytometry [Elektronische Daten] [E. Schulte, H. Lyon, P. Hoyer] Summary: For simultaneous cytophotometric measurement of DNA and RNA, the standardized Methyl Green-Pyronin Y technique is an obvious choice. It is, however, first necessary to correlate the uptake of Pyronin Y to the staining intensity of RNA. The material consisted of paraffin sections of formalin- or Carnoy-fixed rat liver. The sections were pretreated with water, buffer, deoxyribonuclease, ribonuclease, or both enzymes in sequence, and stained with the standardized Methyl Green-Pyronin Y procedure, Gallocyanin chromalum, or the Feulgen analtion. Sections stained directly without pretreatment served as controls. Staining intensities were measured with an image analyser for cell nuclei, nucleoli and cytoplasm. After deoxyribonuclease treatment, nuclear staining intensity with Methyl Green, Gallocyanin chromalum, and Schiff's reagent dropped nearly to zero. The same was seen for both nucleoli and cytoplasm with Pyronin Y and Gallocyanin chromalum after ribonuclease treatment. Staining intensity of Pyronin Y correlated directly with that of Gallocyanin chromalum for nucleoli and cytoplasm. After ribonuclease treatment, a direct correlation was found between the nuclear staining intensity of Methyl Green and nuclear absorption of Gallocyanin chromalum. We conclude that the standardized Methyl Green-Pyronin Y stain is reliable for the simultaneous quantitative assessment of both RNA and DNA. The simplicity of this technique makes it a valuable tool even for daily routine. Chapman & Hall, 1992 Schulte E. Anatomische Anstalt, Ludwig-Maximilians-Universität, Pettenkoferstrasse 11, D-8000, München 2, Germany aut Lyon H. Hvidovre Hospital, Department of Pathology, University of Copenhagen, DK-2640, Hvidovre, Denmark aut Hoyer P. Department of Medical Anatomy A, The Panum Institute, University of Copenhagen, DK-2200, Copenhagen, Denmark aut The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 305-310 0018-2214 24:6<305 1992 24 10735 https://doi.org/10.1007/BF01046161 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046161 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schulte E. Anatomische Anstalt, Ludwig-Maximilians-Universität, Pettenkoferstrasse 11, D-8000, München 2, Germany aut NATIONALLICENCE 700 1- Lyon H. Hvidovre Hospital, Department of Pathology, University of Copenhagen, DK-2640, Hvidovre, Denmark aut NATIONALLICENCE 700 1- Hoyer P. Department of Medical Anatomy A, The Panum Institute, University of Copenhagen, DK-2200, Copenhagen, Denmark aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 305-310 0018-2214 24:6<305 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer