caa a22 4500 469030283 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF01046164 doi (NATIONALLICENCE)springer-10.1007/BF01046164 Histochemical analysis of rat testicular glycoconjugates. 2. β-galactosyl residues in O- and N-linked glycans in seminiferous tubules [Elektronische Daten] [Carolyn Jones, Christopher Morrison, Robert Stoddart] Summary: Rat testes have been examined with a panel of lectins that bind specifically to oligosaccharide sequences having terminal or subterminal β-galactosyl residues in O-linked glycans, or in the outer chains of complex N-linked glycans:Arachis hypogaea (peanut, AHA),Erythrina cristagalli (coral tree, ECA),Ricinus communis (castor bean, RCA120) andAbrus precatorius (jequirity bean, APA) agglutinins. Pretreatment of sections with neuraminidase, β-galactosidase and removal of alkali-labile O-linked sequences by β-elimination allowed the structure of these glycans to be further explored. In spermatogonia and spermatocytes there was little evidence of glycans terminating in β-galactosyl residues, although these were present at non-reducing terminals as sialylgalactosides. The acrosome contained two subsets of O-linked glycans terminating in sialylgalactosides, while the nuclear cap showed at least two subsets of N-linked sialylgalactosyl as well as O-linked glycans. Spermatozoa exhibited minor changes in the pattern of glycosylation, although the overall pattern of β-galactosyl expression was similar. Binding to Sertoli cells showed the presence of some unsubstituted β-galactosyl terminals on O-linked glycans but few such N-linked residues, while terminal β-galactosides were scanty in tubular basement membranes. Chapman & Hall, 1992 Jones Carolyn Department of Pathological Sciences, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut Morrison Christopher Department of Immunology, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut Stoddart Robert Department of Pathological Sciences, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 327-336 0018-2214 24:6<327 1992 24 10735 https://doi.org/10.1007/BF01046164 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046164 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jones Carolyn Department of Pathological Sciences, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut NATIONALLICENCE 700 1- Morrison Christopher Department of Immunology, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut NATIONALLICENCE 700 1- Stoddart Robert Department of Pathological Sciences, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/6(1992-06-01), 327-336 0018-2214 24:6<327 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 469030216 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer