caa a22 4500 469030348 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF01047585 doi (NATIONALLICENCE)springer-10.1007/BF01047585 Differential staining of glycosaminoglycans in the predentine and dentine of rat incisor using Cuprolinic Blue at various magnesium chloride concentrations [Elektronische Daten] [M. Goldberg, D. Septier] Summary: Rat incisors were fixed with a solution of 0.05% Cuprolinic Blue and 2.5% glutaraldehyde in the presence of various concentrations of MgCl2 according to the critical electrolyte concentration (CEC) principle. This method allows glycosaminoglycans (GAG) to be properly preserved and visualized. Small granules were stained by the cationic dye in the predentine in the absence of MgCl2. These granules grew in size and became more electron-dense when the concentration of the electrolyte was increased. Larger ribbon-like structures and granules were seen when 0.3M MgCl2 was used. In the dentine, tiny dots in close association with the surface of the collagen fibres, or their periodic striations, were positively stained. A thick electron-dense band located on the dentine side at the predentine-dentine junction was seen both with and without 0.05 M MgCl2. With higher concentrations of the electrolyte (0.1-0.3 M), this band was reduced to a very thin line located at the border of the dentine, along with mineralizing collagen fibres. This demonstrated the presence of GAG at the dentine surface and therefore indicated that GAG may play a role as nucleator agent. Chapman & Hall, 1992 Goldberg M. Laboratoire de Biologie et Biomatériaux du Milieu Buccal et Osseux, groupe: Matrices Extracellulaires et Biominéralisations, Faculté de Chirurgie Dentaire (Paris V), I rue Maurice Arnoux, 92120, Montrouge, France aut Septier D. Laboratoire de Biologie et Biomatériaux du Milieu Buccal et Osseux, groupe: Matrices Extracellulaires et Biominéralisations, Faculté de Chirurgie Dentaire (Paris V), I rue Maurice Arnoux, 92120, Montrouge, France aut The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 648-654 0018-2214 24:9<648 1992 24 10735 https://doi.org/10.1007/BF01047585 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01047585 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goldberg M. Laboratoire de Biologie et Biomatériaux du Milieu Buccal et Osseux, groupe: Matrices Extracellulaires et Biominéralisations, Faculté de Chirurgie Dentaire (Paris V), I rue Maurice Arnoux, 92120, Montrouge, France aut NATIONALLICENCE 700 1- Septier D. Laboratoire de Biologie et Biomatériaux du Milieu Buccal et Osseux, groupe: Matrices Extracellulaires et Biominéralisations, Faculté de Chirurgie Dentaire (Paris V), I rue Maurice Arnoux, 92120, Montrouge, France aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 648-654 0018-2214 24:9<648 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer