caa a22 4500 469030356 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF01047588 doi (NATIONALLICENCE)springer-10.1007/BF01047588 Calbindin D-28k-immunoreactivity in rat muscle spindles during postnatal maturation and after denervation [Elektronische Daten] [Marke Hietanen-Peltola, Markku Pelto-Huikko, Leena Rechardt, Tomas Hökfelt] Summary: Calbindin D-28k-immunoreactivity has been demonstrated in some of the intrafusal muscle fibres and in the capsule of adult rat muscle spindles. In this study, the immunocytochemical localization of calbindin D-28k in the muscle spindles of triceps surae muscle was studied during postnatal maturation and after denervation. In young rats calbindin D-28k-immunoreactivity was seen in a few intrafusal fibres, first at the age of 4 days. At the 7th day, three calbindin D-28k-immunoreactive fibres and one unlabelled fibre were seen in most muscle spindles, as in adult rats. The spindle capsule and perineurial sheath of nerves were first seen to exhibit calbindin D-28k immunoreactivity at the age of 14 days, and thereafter the localization of calbinding D-28k-like immunoreactivity was similar to that in adult rats. After denervation, calbindin D-28k-immunoreactivity remained in intrafusal muscle fibres and the spindle capsule for a long period. After two months of denervation, calbindin D-28k immunoreactivity could still be seen in the spindle capsule, but the intrafusal fibres were not labelled. The innervation is known to have trophic effects on the intrafusal fibres. The present findings suggest that the expression of calbindin D-28k-immunoreactivity in maturating muscle spindles may be induced by the developing innervation. The decrease of calbindin D-28k-immunoreactivity in intrafusal fibres after denervation may be due to the loss of trophic factors released by the nerves. Chapman & Hall, 1992 Hietanen-Peltola Marke Department of Biomedical Sciences, University of Tampere, 33101, Tampere, Finland aut Pelto-Huikko Markku Department of Biomedical Sciences, University of Tampere, 33101, Tampere, Finland aut Rechardt Leena Department of Biomedical Sciences, University of Tampere, 33101, Tampere, Finland aut Hökfelt Tomas Department of Neurobiology and Histology, Karolinska Institute, Stockholm, Sweden aut The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 673-678 0018-2214 24:9<673 1992 24 10735 https://doi.org/10.1007/BF01047588 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01047588 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hietanen-Peltola Marke Department of Biomedical Sciences, University of Tampere, 33101, Tampere, Finland aut NATIONALLICENCE 700 1- Pelto-Huikko Markku Department of Biomedical Sciences, University of Tampere, 33101, Tampere, Finland aut NATIONALLICENCE 700 1- Rechardt Leena Department of Biomedical Sciences, University of Tampere, 33101, Tampere, Finland aut NATIONALLICENCE 700 1- Hökfelt Tomas Department of Neurobiology and Histology, Karolinska Institute, Stockholm, Sweden aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 673-678 0018-2214 24:9<673 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer