caa a22 4500 469030364 CHVBK 20180323132741.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF01047586 doi (NATIONALLICENCE)springer-10.1007/BF01047586 AMC-anti-FITC conjugates: novel reagents for amplified immunochemical techniques. Immunofluorescent staining of human fibroblasts [Elektronische Daten] [R. Abuknesha, H. Al-Mazeedi, R. Price] Summary: Fluorescein antibodies were labelled with 7-aminocoumarin (AMC) derivatives, the 3-acetic acid and the 3-propionic acidN-hydroxysuccinimide esters. The labelled antibodies were used in conjunction with fluorescein isothiocyanate (FITC) and carboxyfluorescein-conjugated primary and secondary antibodies to develop novel immunofluorescent staining procedures. These methods combine the advantages of the fluorescence properties of AMC and the ready availability of FITC-labelled antisera to provide an amplified fluorescence signal as well as overcoming the photobleaching problems in FITC staining. The method is easy to perform and is expected to make an important contribution to the improvement of the quality of staining achieved with immunofluorescence. Details of the procedure used to stain human fibroblasts with antifibronectin antibodies are reported in order to illustrate the method. Chapman & Hall, 1992 Abuknesha R. Bioelectrochemistry and Biosensors Group, Department of Chemistry, King's College, Campden Hill Road, W8 7AH, London, UK aut Al-Mazeedi H. Biochemistry Section, Biomolecular Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK aut Price R. Biochemistry Section, Biomolecular Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK aut The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 655-662 0018-2214 24:9<655 1992 24 10735 https://doi.org/10.1007/BF01047586 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01047586 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abuknesha R. Bioelectrochemistry and Biosensors Group, Department of Chemistry, King's College, Campden Hill Road, W8 7AH, London, UK aut NATIONALLICENCE 700 1- Al-Mazeedi H. Biochemistry Section, Biomolecular Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK aut NATIONALLICENCE 700 1- Price R. Biochemistry Section, Biomolecular Sciences Division, King's College, Campden Hill Road, W8 7AH, London, UK aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 655-662 0018-2214 24:9<655 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer