caa a22 4500 469030372 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF01047589 doi (NATIONALLICENCE)springer-10.1007/BF01047589 A chromogranin peptide is co-stored with insulin in the human pancreatic islet B-cell granules [Elektronische Daten] [A. Lukinius, E. Wilander, B. Eriksson, K. Öberg] Summary: The immunoreaction of a rabbit chromogranin A and B antiserum was studied in normal human pancreatic islets. By examination of consecutive light microscopical sections, it was revealed that, at high antiserum concentrations (1:2000 or less), the whole islet area was heavily labelled, although the peripheral glucagon (A)-cells were the most intense in their immunoreaction. At low antiserum concentrations (1:4000 or more) the A-cells still showed the same intense labelling reaction, but the central B-cells were weakly labelled. Electron microscopically, reactivity towards the chromogranin A and B antiserum and the monoclonal insulin antibodies was present in the same central electron-dense core of the B-cell secretory granules, as demonstrated after application of the immunogold technique at different antibody dilutions. In the A-cells, the chromogranin immunoreactivity was concentrated at the peripheral mantle of the secretory granules. The D-cell granules showed a weak immunolabelling. Examination of human islets with the monoclonal chromogranin A antibody LK2H10 revealed immunogold labelling only in the peripheal mantle of the A-cell granules, while the B-cell granules were unlabelled. The present results show that a chromogranin peptide is co-stored with insulin the in normal human B-cell secretory granules. Although the exact composition of this B-cell chromogranin is unknown, it is not identical to that of the chromogranin A present in the A-cell granules. Chapman & Hall, 1992 Lukinius A. Department of Pathology, University Hospital, S-751 85, Uppsala, Sweden aut Wilander E. Department of Pathology, University Hospital, S-751 85, Uppsala, Sweden aut Eriksson B. Department of Internal Medicine, University Hospital, S-751 85, Uppsala, Sweden aut Öberg K. Department of Internal Medicine, University Hospital, S-751 85, Uppsala, Sweden aut The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 679-684 0018-2214 24:9<679 1992 24 10735 https://doi.org/10.1007/BF01047589 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01047589 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lukinius A. Department of Pathology, University Hospital, S-751 85, Uppsala, Sweden aut NATIONALLICENCE 700 1- Wilander E. Department of Pathology, University Hospital, S-751 85, Uppsala, Sweden aut NATIONALLICENCE 700 1- Eriksson B. Department of Internal Medicine, University Hospital, S-751 85, Uppsala, Sweden aut NATIONALLICENCE 700 1- Öberg K. Department of Internal Medicine, University Hospital, S-751 85, Uppsala, Sweden aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 679-684 0018-2214 24:9<679 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer