caa a22 4500 469030380 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF01047590 doi (NATIONALLICENCE)springer-10.1007/BF01047590 Cellular heterogeneity in human transitional cell carcinoma: an anlysis of optical properties and lectin binding [Elektronische Daten] [Glen Ward, Stephen Stewart, Gus Dotsikas, Gerald Price, William Mackillop] Summary: Flow cytometry was used to measure the binding of a panel of ten fluorescein isothiocyanate(FITC)-conjugated lectins to fifteen samples of normal and neoplastic human urothelium. Concurrent measurement of light scattering and fluorescence permitted the quantification of lectin binding to cellular subpopulations defined by their light-scattering properties. In normal urothelium, we previously demonstrated levels of lectin binding to the cellular subpopulations derived from the superficial and intermediate cell layers which were higher than levels which bound to the subpopulation derived from the basal cell layer (Wardet al., 1987). This difference was most marked withMaclura pomifera agglutinin (MPA),Ricinus communis agglutinin (RCA) andUlex europus agglutinin (UEA). We now report a similar correlation between the degree of differentiation of a cellular subpopulation and the level of lectin binding in human transitional cell carcinomas (TCCs). Morphological differentiation in human TCCs is accompanied by alterations in cell-surface carbohydrates which are similar to those which accompany cellular differentiation in the corresponding normal tissue. No systematic difference in lectin binding was observed between the corresponding subpopulations of normal and neoplastic urothelial cells. Chapman & Hall, 1992 Ward Glen McGill Cancer Centre, McGill University, Montreal, Quebec, Canada aut Stewart Stephen McGill Cancer Centre, McGill University, Montreal, Quebec, Canada aut Dotsikas Gus McGill Cancer Centre, McGill University, Montreal, Quebec, Canada aut Price Gerald McGill Cancer Centre, McGill University, Montreal, Quebec, Canada aut Mackillop William McGill Cancer Centre, McGill University, Montreal, Quebec, Canada aut The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 685-694 0018-2214 24:9<685 1992 24 10735 https://doi.org/10.1007/BF01047590 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01047590 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ward Glen McGill Cancer Centre, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Stewart Stephen McGill Cancer Centre, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Dotsikas Gus McGill Cancer Centre, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Price Gerald McGill Cancer Centre, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Mackillop William McGill Cancer Centre, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 685-694 0018-2214 24:9<685 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer