caa a22 4500 469030399 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF01047584 doi (NATIONALLICENCE)springer-10.1007/BF01047584 The evolution of proteinase substrates with special reference to dipeptidylpeptidase IV [Elektronische Daten] [Robert Smith, Carmen Reynolds, Elizabeth Elder] Summary: The design and development of specific substrates for proteolytic enzymes is reviewed. Particular attention is given to substrates containing the leaving groups 4-methoxy-2-naphthylamide (MNA) and 7-amino-4-trifluoromethylcoumarin (AFC). The MNA substrates are used for histochemical and cytochemical purposes, and they yield a coloured final reaction product when azo-coupled with a diazonium salt, an osmiophilic product for electron microscopy when coupled with hexazotized Pararosaniline, or a fluorescent final reaction product when coupled with 5-nitrosalicylaldehyde. AFC substrates are considerably more sensitive, and they yield the fluorescent product AFC after enzymatic cleavage of the substrate. AFC is not sufficiently water-insoluble to allow (intra)cellular localization, but AFC substrates are successfully used for incubations in microwells (Immu-Probe technique) and for the demonstration of banding patterns after gel electrophoresis (enzyme-directed overlay membrane technique). The methods are discussed with the example of the elucidation of the role of dipeptidylpeptidase IV in autoimmune diseases. Chapman & Hall, 1992 Smith Robert Prototek, Inc., 6491 Sierra Lane, 94568, Dublin, CA, USA aut Reynolds Carmen Prototek, Inc., 6491 Sierra Lane, 94568, Dublin, CA, USA aut Elder Elizabeth Prototek, Inc., 6491 Sierra Lane, 94568, Dublin, CA, USA aut The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 637-647 0018-2214 24:9<637 1992 24 10735 https://doi.org/10.1007/BF01047584 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01047584 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smith Robert Prototek, Inc., 6491 Sierra Lane, 94568, Dublin, CA, USA aut NATIONALLICENCE 700 1- Reynolds Carmen Prototek, Inc., 6491 Sierra Lane, 94568, Dublin, CA, USA aut NATIONALLICENCE 700 1- Elder Elizabeth Prototek, Inc., 6491 Sierra Lane, 94568, Dublin, CA, USA aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/9(1992-09-01), 637-647 0018-2214 24:9<637 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer