caa a22 4500 469030429 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF01089102 doi (NATIONALLICENCE)springer-10.1007/BF01089102 Murray Graeme Department of Pathology, University of Aberdeen, AB9 2ZD, Aberdeen, UK aut Enzyme histochemistry and immunohistochemistry with freeze-dried or freeze-substituted resin-embedded tissue [Elektronische Daten] [Graeme Murray] Summary: Freeze-drying or freeze-substitution, combined with low-temperature resin-embedding, represents a new approach to the optimum preservation of tissue for enzyme histochemistry and immunohistochemistry. This method, which avoids tissue fixation, combines excellent tissue morphology with the preservation of enzyme activity and immunoreactivity and allows high-resolution enzyme histochemical and immunohistochemical studies to be performed. The activity of a wide range of enzymes can be demonstrated in sections of freeze-dried or freeze-substituted resin-embedded tissue. Enzymes are retainedin situ with high activity, accurate localization and no diffusion. Immunohistochemical studies can also be performed on resin sections, and antigens—especially labile antigens — are immobilizedin situ without denaturation and can be demonstrated with high sensitivity and accurately localized. This method allows the localization and distribution of enzymes and antigens to be studied in relation to excellent histological and cytological detail. Chapman & Hall, 1992 The Histochemical Journal Kluwer Academic Publishers 24/7(1992-07-01), 399-408 0018-2214 24:7<399 1992 24 10735 https://doi.org/10.1007/BF01089102 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01089102 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Murray Graeme Department of Pathology, University of Aberdeen, AB9 2ZD, Aberdeen, UK aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/7(1992-07-01), 399-408 0018-2214 24:7<399 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer