caa a22 4500 469030453 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF01089103 doi (NATIONALLICENCE)springer-10.1007/BF01089103 Hirabayashi Yoshifumi Department of Anatomy, Nagoya City University Medical School, 467, Nagoya, Japan aut Light-microscopic detection of acidic glycoconjugates with sensitized diamine procedures [Elektronische Daten] [Yoshifumi Hirabayashi] Summary: To enhance the efficiency and specificity of diamine methods in light microscopy, these methods were sensitized by sliver enhancement in combination with trichloro(ethylene) platinate (KTP). The sensitized diamine methods consisted of a diamine (high or low iron diamine: HID or LID), KTP, borohydride reduction (BH) and a physical development (PD) sequence. The new methods have been successfully applied to routinely prepared tissue sections obtained from rat organs, such as salivary glands, stomach, colon, kidney, lung and trachea. In the tissues subjected to the sensitized diamine methods, weakly diamine-stained histological structures exhibited vivid positive reactions. The combined sensitized diamine methods and selective procedures, such as enzyme digestion and chemical modification, have substantiated that these methods were of sufficient efficiency and specificity. Chapman & Hall, 1992 The Histochemical Journal Kluwer Academic Publishers 24/7(1992-07-01), 409-418 0018-2214 24:7<409 1992 24 10735 https://doi.org/10.1007/BF01089103 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01089103 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Hirabayashi Yoshifumi Department of Anatomy, Nagoya City University Medical School, 467, Nagoya, Japan aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/7(1992-07-01), 409-418 0018-2214 24:7<409 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer