caa a22 4500 469030461 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19920701xx s 000 0 eng 10.1007/BF01089104 doi (NATIONALLICENCE)springer-10.1007/BF01089104 Cationic colloidal gold — a probe for light- and electron-microscopic characterization of acidic glycoconjugates using poly- l -lysine gold complex [Elektronische Daten] [Nobuyuki Kashio, Shinichiro Tsuyama, Kaori Ihida, Fusayoshi Murata] Summary: Cationic colloidal gold (CCG) was used to characterize acidic glycoconjugates in semithin and ultrathin sections of rat large intestine and salivary glands embedded in hydrophilic Lowicryl K4M resin. It was prepared from poly-l-lysine and 10 nm colloidal gold solution. The staining of CCG in semithin sections was amplified after photochemical silver reaction using silver acetate as a silver ion donor and examined under bright-field and epi-illumination microscopy. CCG adjusted to various pH levels was tested on various rat tissues whose histochemical characteristics with regard to acidic glycoconjugates are well known. At pH 2.5 CCG labelled tissues containing sialylated and sulphated acidic glycoconjugates such as the apical cell surface, mucous cells in the distal and proximal colon, and acinar cells of the sublingual gland. In contrast, CCG at pH 1.0 labelled tissues containing sulphated acidic glycoconjugates such as mucous cells in the upper crypt of the proximal colon and mucous cells in the whole crypt of the distal colon. This specificity of CCG was verified by the alteration of CCG staining following several types of cytochemical pretreatment. These results were further confirmed by electron microscopy. CCG staining is thus a useful postembedding procedure for the characterization of acidic glycoconjugates at both the light- and electron-microscopic levels. Chapman & Hall, 1992 Kashio Nobuyuki Department of Anatomy, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, 890, Kagoshima, Japan aut Tsuyama Shinichiro Department of Anatomy, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, 890, Kagoshima, Japan aut Ihida Kaori Department of Anatomy, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, 890, Kagoshima, Japan aut Murata Fusayoshi Department of Anatomy, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, 890, Kagoshima, Japan aut The Histochemical Journal Kluwer Academic Publishers 24/7(1992-07-01), 419-430 0018-2214 24:7<419 1992 24 10735 https://doi.org/10.1007/BF01089104 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01089104 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kashio Nobuyuki Department of Anatomy, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, 890, Kagoshima, Japan aut NATIONALLICENCE 700 1- Tsuyama Shinichiro Department of Anatomy, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, 890, Kagoshima, Japan aut NATIONALLICENCE 700 1- Ihida Kaori Department of Anatomy, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, 890, Kagoshima, Japan aut NATIONALLICENCE 700 1- Murata Fusayoshi Department of Anatomy, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, 890, Kagoshima, Japan aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/7(1992-07-01), 419-430 0018-2214 24:7<419 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer