caa a22 4500 469030496 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF01046498 doi (NATIONALLICENCE)springer-10.1007/BF01046498 Calcium localization in the shell-forming tissue of the freshwater snail, Biomphalaria glabrata [Elektronische Daten] a comparative study of various methods for localizing calcium [Ulrich Bielefeld, Karl Zierold, Karl Körtje, Wilhelm Becker] Summary: The routes calcium might take across the mantle to the shell have been investigated with various electron-microscopical techniques in the freshwater snailBiomphalaria glabrata (Planorbidae, Basommatophora). In chemically-fixed tissue, calcium was precipitated with a tannic acid-antimonate technique in predominantly the intercellular spaces of the outer mantle epithelium and the interstitium below it. Some vacuoles of the outer mantle epithelium and one type of mucus cell in the inner mantle epithelium also contained precipitate. The presence of calcium in the precipitates was proved by electron energy loss spectroscopy combined with electron spectroscopic imaging. Incubation with lead acetate and uranyl acetate revealed binding-sites for calcium in the intercellular spaces of the epithelia interstitium and the mucus cells of the inner mantle epithelium. Precipitates were also seen after all incubations in the calcium spherites of the connective tissue. The concentrations of calcium and other elements were analysed in freeze-dried ultrathin sections of cryofixed mantle tissue by means of energy-dispersive X-ray microanalysis. Only in mitochondria of the musculature could high amounts of calcium and phosphorous be detected. Chapman & Hall, 1992 Bielefeld Ulrich Zoologisches Institut und Zoologisches Museum, Martin-Luther-King Platz 3, D-2000, Hamburg 13, Germary aut Zierold Karl Max-Planck-Institut für Systemphysiologie, Rheinlanddamm 201, D-4600, Dortmund-1, Germany aut Körtje Karl Zoologisches Institut, Universität Stuttgart-Hohenheim, Garbenstraße, D-7000, Stuttgart, Germany aut Becker Wilhelm Zoologisches Institut und Zoologisches Museum, Martin-Luther-King Platz 3, D-2000, Hamburg 13, Germary aut The Histochemical Journal Kluwer Academic Publishers 24/12(1992-12-01), 927-938 0018-2214 24:12<927 1992 24 10735 https://doi.org/10.1007/BF01046498 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046498 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bielefeld Ulrich Zoologisches Institut und Zoologisches Museum, Martin-Luther-King Platz 3, D-2000, Hamburg 13, Germary aut NATIONALLICENCE 700 1- Zierold Karl Max-Planck-Institut für Systemphysiologie, Rheinlanddamm 201, D-4600, Dortmund-1, Germany aut NATIONALLICENCE 700 1- Körtje Karl Zoologisches Institut, Universität Stuttgart-Hohenheim, Garbenstraße, D-7000, Stuttgart, Germany aut NATIONALLICENCE 700 1- Becker Wilhelm Zoologisches Institut und Zoologisches Museum, Martin-Luther-King Platz 3, D-2000, Hamburg 13, Germary aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/12(1992-12-01), 927-938 0018-2214 24:12<927 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer