caa a22 4500 469030526 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF01046499 doi (NATIONALLICENCE)springer-10.1007/BF01046499 The mapping of natural boron in histological sections of mouse tissues by the use of neutron-capture radiography [Elektronische Daten] [Maria Laurent-Pettersson, Bertrand Delpech, Michel Thellier] Summary: Neutron-capture radiography has been applied to the mapping of natural boron in mouse histological sections. The method is based on the fact that the stable isotope boron-10 has an extremely large cross-section for the thermal-neutron reaction10B(n, α)7Li. The local tissue boron concentrations were evaluated from the corresponding track densities of the nuclear reaction using an equation derived from a theoretical model of the system. The adjustable parameters of this equation were determined in two separate calibration experiments using standardized samples prepared by the addition of known amounts of boron. Interference from other nuclides engaged in nuclear reactions with thermal neutrons was also estimated. In the present experimental conditions the natural boron lower detection limit was 0.03 p.p.m. (fresh weight), and the spatial resolution was of the order of a few micrometers. Boron concentrations in mouse serum and urine were close to 0.22 and 0.57 μg ml−1, respectively. In the solid mouse tissues-liver, heart, brain, muscle and spleen-the concentration was usually low, ranging from 0.12 to 0.16 p.p.m. (fresh weight). They were significantly higher in the kidney, especially in the papilla (6.2 p.p.m. fresh weight). Apart from the kidney papilla, where boron was particularly concentrated in wall tubules, the boron distribution in most tissues appeared to be practically homogeneous. Natural boron, and even more so its enriched stable isotope10B, appear as good candidates for molecular labelling using non-radioactive tracers. Chapman & Hall, 1992 Laurent-Pettersson Maria Laboratoire d'Oncologie Moléculaire, Centre Henri-Becquerel, rue d'Amiens, F-76038, Rouen Cedex, France aut Delpech Bertrand Laboratoire d'Oncologie Moléculaire, Centre Henri-Becquerel, rue d'Amiens, F-76038, Rouen Cedex, France aut Thellier Michel Laboratoire Echanges Cellulaires, URA 203 CNRS, Faculté des Sciences et Techniques, BP 118, F-76134, Mont-Saint-Aignan Cedex, France aut The Histochemical Journal Kluwer Academic Publishers 24/12(1992-12-01), 939-950 0018-2214 24:12<939 1992 24 10735 https://doi.org/10.1007/BF01046499 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01046499 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Laurent-Pettersson Maria Laboratoire d'Oncologie Moléculaire, Centre Henri-Becquerel, rue d'Amiens, F-76038, Rouen Cedex, France aut NATIONALLICENCE 700 1- Delpech Bertrand Laboratoire d'Oncologie Moléculaire, Centre Henri-Becquerel, rue d'Amiens, F-76038, Rouen Cedex, France aut NATIONALLICENCE 700 1- Thellier Michel Laboratoire Echanges Cellulaires, URA 203 CNRS, Faculté des Sciences et Techniques, BP 118, F-76134, Mont-Saint-Aignan Cedex, France aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/12(1992-12-01), 939-950 0018-2214 24:12<939 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer