caa a22 4500 469030542 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01047466 doi (NATIONALLICENCE)springer-10.1007/BF01047466 The use of hyaluronidase and glucosidase to remove mucus from the rat middle ear cavities for SEM studies [Elektronische Daten] [Magnus Nilsson, Sten Hellström, Ulla Hedlund] Summary: To conduct SEM studies on epithelium containing mucus-producing cells it is essential to remove the mucus which normally obscures the epithelial surface. This study presents a method which effectively removes the covering layer of mucus in the rat middle ear. Healthy Sprague-Dawley rats were decapitated and the middle ears dissected free. Incubation and agitation of the middle ear specimens in hyaluronidase (50 IE ml−1) and/or glucosidase (8%) removed the mucus from the middle ear cavity without altering the surface structures. It was also revealed that substances such as polyvinyl-pyrrolidone (PVP) (used to increase the colloid osmotic pressure of, e.g., the fixative solution) must be omitted when preparing ciliated specimens for SEM. Chapman & Hall, 1992 Nilsson Magnus Department of Anatomy, University of Umeå, S-901 87, Umeå, Sweden aut Hellström Sten Department of Anatomy, University of Umeå, S-901 87, Umeå, Sweden aut Hedlund Ulla Department of Anatomy, University of Umeå, S-901 87, Umeå, Sweden aut The Histochemical Journal Kluwer Academic Publishers 24/3(1992-03-01), 166-169 0018-2214 24:3<166 1992 24 10735 https://doi.org/10.1007/BF01047466 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01047466 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nilsson Magnus Department of Anatomy, University of Umeå, S-901 87, Umeå, Sweden aut NATIONALLICENCE 700 1- Hellström Sten Department of Anatomy, University of Umeå, S-901 87, Umeå, Sweden aut NATIONALLICENCE 700 1- Hedlund Ulla Department of Anatomy, University of Umeå, S-901 87, Umeå, Sweden aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/3(1992-03-01), 166-169 0018-2214 24:3<166 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer