caa a22 4500 469030569 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01047461 doi (NATIONALLICENCE)springer-10.1007/BF01047461 The assessment of cellular proliferation by immunohistochemistry: A review of currently available methods and their applications [Elektronische Daten] [Cermen Yu, Amanda Woods, David Levison] Summary: Immunohistochemical methods using antibodies to cell cycle-related antigens may be used as a means of assessing various aspects of proliferation in tissue, and have the important advantage of preserving the spatial orientation of proliferating cells in histological sections. Currently, the most widely available antibodies for this purpose are antibodies to bromodeoxyuridine (BrdU), Ki67 and antibodies to proliferating cell nuclear antigen (PCNA). BrdU is a thymidine analogue incorporated during the S phase of the cell cycle, which can be introduced by ‘in vivo' administration or by ‘in vitro' incubation, and monoclonal antibodies are available to display its localization. Ki67 demonstrates a nuclear antigen expressed in all phases of the cell cycle, except G0 and early G1, but can only be applied to frozen tissue. PCNA is a nuclear antigen which is essential for DNA synthesis, two commercially available antibodies to PCNA work in paraffin-embedded tissue, but may have different staining characteristics under different conditions of fixation. The main advantages and disadvantages of these different techniques are discussed, together with their main applications to date. Chapman & Hall, 1992 Yu Cermen Department of Histopathology, UMDS, Guy's Hospital, St. Thomas's St., SE1 9RT, London, UK aut Woods Amanda Department of Histopathology, UMDS, Guy's Hospital, St. Thomas's St., SE1 9RT, London, UK aut Levison David Department of Histopathology, UMDS, Guy's Hospital, St. Thomas's St., SE1 9RT, London, UK aut The Histochemical Journal Kluwer Academic Publishers 24/3(1992-03-01), 121-131 0018-2214 24:3<121 1992 24 10735 https://doi.org/10.1007/BF01047461 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01047461 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Cermen Department of Histopathology, UMDS, Guy's Hospital, St. Thomas's St., SE1 9RT, London, UK aut NATIONALLICENCE 700 1- Woods Amanda Department of Histopathology, UMDS, Guy's Hospital, St. Thomas's St., SE1 9RT, London, UK aut NATIONALLICENCE 700 1- Levison David Department of Histopathology, UMDS, Guy's Hospital, St. Thomas's St., SE1 9RT, London, UK aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/3(1992-03-01), 121-131 0018-2214 24:3<121 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer