caa a22 4500 469030607 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01047462 doi (NATIONALLICENCE)springer-10.1007/BF01047462 Immunogold labelling is a quantitative method as demonstrated by studies on aminopeptidase N in microvillar membrane vesicles [Elektronische Daten] [Gert Hansen, Lise-Lotte Wetterberg, Hans Sjöström, Ove Norén] Summary: Microvillar membrane vesicle preparations with varying content of aminopeptidase N were prepared from enterocytes of the pig small intestine. Postembedding immunogold labelling of aminopeptidase N was performed on these glutaraldehyde/paraformaldehyde-fixed, osmium tetroxide-treated and Epon-embedded microvillar membrane vesicles. The number of gold particles per micrometre microvillar membrane (labelling intensity) was calculated and compared to the corresponding enzymatic activity. A very close relationship was found between labelling intensity and aminopeptidase N activity, demonstrating that postembedding immunogold labelling can be used quantitatively. Chapman & Hall, 1992 Hansen Gert Department of Biochemistry C, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, 2200, Copenhagen N, Denmark aut Wetterberg Lise-Lotte Department of Biochemistry C, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, 2200, Copenhagen N, Denmark aut Sjöström Hans Department of Biochemistry C, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, 2200, Copenhagen N, Denmark aut Norén Ove Department of Biochemistry C, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, 2200, Copenhagen N, Denmark aut The Histochemical Journal Kluwer Academic Publishers 24/3(1992-03-01), 132-136 0018-2214 24:3<132 1992 24 10735 https://doi.org/10.1007/BF01047462 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01047462 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hansen Gert Department of Biochemistry C, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, 2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Wetterberg Lise-Lotte Department of Biochemistry C, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, 2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Sjöström Hans Department of Biochemistry C, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, 2200, Copenhagen N, Denmark aut NATIONALLICENCE 700 1- Norén Ove Department of Biochemistry C, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, 2200, Copenhagen N, Denmark aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/3(1992-03-01), 132-136 0018-2214 24:3<132 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer