caa a22 4500 469030615 CHVBK 20180323132742.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF01047463 doi (NATIONALLICENCE)springer-10.1007/BF01047463 Quantification of the cellular proliferation on freshly dispersed cells from rat anterior pituitaries after in vivo and in vitro labelling with bromodeoxyuridine [Elektronische Daten] [S. Carbajo, F. Gonzalez Del Pozo, E. Carbajo-Perez] Summary: The labelling index i.e., the proportion of cells in S phase of the cell cycle, has been calculated in cytospin preparations of rat anterior pituitary cells after labelling eitherin vivo orin vitro with the thymidine analogue bromodeoxyuridine (BrdU). The aims of this work were (1) to check whether enzymatic digestion interferes with the incorporation of BrdU into S phase cells and/or whether it has any deletereous effect on the immunohistochemical detection of cells that have already incorporated BrdU, and (2) to check the viability of simultaneous staining for BrdU and markers for the different types of pituitary cells in the cytospins. No statistical difference was found between the labelling index afterin vivo orin vitro labelling with BrdU. Identification of doubly-immunostained cells was straightforward and up to 40% of BrdU-labelled cells were immunopositive for pituitary hormones. It is suggested that cytospin preparations from biopsy samples may be used to study cellular proliferation without exposing the patient to the hazardous effects of BrdU infusion and without the interference of cell culture methods. Chapman & Hall, 1992 Carbajo S. Department of Anatomy and Histology, Faculty of Medicine, University of Salamanca, Campo Charro s/n, 37007, Salamanca, Spain aut Gonzalez Del Pozo F. Department of Anatomy and Histology, Faculty of Medicine, University of Salamanca, Campo Charro s/n, 37007, Salamanca, Spain aut Carbajo-Perez E. Department of Anatomy and Histology, Faculty of Medicine, University of Salamanca, Campo Charro s/n, 37007, Salamanca, Spain aut The Histochemical Journal Kluwer Academic Publishers 24/3(1992-03-01), 137-143 0018-2214 24:3<137 1992 24 10735 https://doi.org/10.1007/BF01047463 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01047463 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carbajo S. Department of Anatomy and Histology, Faculty of Medicine, University of Salamanca, Campo Charro s/n, 37007, Salamanca, Spain aut NATIONALLICENCE 700 1- Gonzalez Del Pozo F. Department of Anatomy and Histology, Faculty of Medicine, University of Salamanca, Campo Charro s/n, 37007, Salamanca, Spain aut NATIONALLICENCE 700 1- Carbajo-Perez E. Department of Anatomy and Histology, Faculty of Medicine, University of Salamanca, Campo Charro s/n, 37007, Salamanca, Spain aut NATIONALLICENCE 773 0- The Histochemical Journal Kluwer Academic Publishers 24/3(1992-03-01), 137-143 0018-2214 24:3<137 1992 24 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer